Exploiting human CD34+ stem cell-conditioned medium for tissue repair

Paul J. Mintz, Kai Wen Huang, Vikash Reebye, Georgios Nteliopoulos, Hong Shiee Lai, Pal Sætrom, Noriyuki Kasahara, Steen Jensen, Madhava Pai, Myrtle Y A Gordon, Stephen B. Marley, Rosemary Behan, Duncan R. Spalding, Abdelali Haoudi, Mohamed Emara, Joanna Nicholls, John J. Rossi, Nagy A. Habib

Research output: Contribution to journalArticle

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Abstract

Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34 + cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble factors in medium conditioned by adherent human CD34 + cells. Thirty-two abundantly secreted factors have been identified, all of which are associated with cell proliferation, survival, tissue repair, and wound healing. The cultured CD34 + cells expressed known stem cell genes such as Nanog, Oct4, Sox2, c-kit, and HoxB4. The conditioned medium containing the secreted factors prevented cell death in liver cells exposed to liver toxin in vitro via inhibition of the caspase-3 signaling pathway. More importantly, in vivo studies using animal models of liver damage demonstrated that injection of the conditioned medium could repair damaged liver tissue (significant reduction in the necroinflammatory activity), as well as enable the animals to survive. Thus, we demonstrate that medium conditioned by human CD34 + cells has the potential for therapeutic repair of damaged tissue in vivo.

Original languageEnglish
Pages (from-to)149-159
Number of pages11
JournalMolecular Therapy
Volume22
Issue number1
DOIs
Publication statusPublished - 1 Jan 2014

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Conditioned Culture Medium
Stem Cells
Liver
Stem Cell Factor
Essential Genes
Microarray Analysis
Caspase 3
Wound Healing
Statistical Factor Analysis
Regeneration
Cultured Cells
Cell Survival
Intercellular Signaling Peptides and Proteins
Cell Death
Animal Models
Cell Proliferation
Cytokines
Injections
Genes
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Genetics
  • Drug Discovery
  • Pharmacology

Cite this

Mintz, P. J., Huang, K. W., Reebye, V., Nteliopoulos, G., Lai, H. S., Sætrom, P., ... Habib, N. A. (2014). Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Molecular Therapy, 22(1), 149-159. https://doi.org/10.1038/mt.2013.194

Exploiting human CD34+ stem cell-conditioned medium for tissue repair. / Mintz, Paul J.; Huang, Kai Wen; Reebye, Vikash; Nteliopoulos, Georgios; Lai, Hong Shiee; Sætrom, Pal; Kasahara, Noriyuki; Jensen, Steen; Pai, Madhava; Gordon, Myrtle Y A; Marley, Stephen B.; Behan, Rosemary; Spalding, Duncan R.; Haoudi, Abdelali; Emara, Mohamed; Nicholls, Joanna; Rossi, John J.; Habib, Nagy A.

In: Molecular Therapy, Vol. 22, No. 1, 01.01.2014, p. 149-159.

Research output: Contribution to journalArticle

Mintz, PJ, Huang, KW, Reebye, V, Nteliopoulos, G, Lai, HS, Sætrom, P, Kasahara, N, Jensen, S, Pai, M, Gordon, MYA, Marley, SB, Behan, R, Spalding, DR, Haoudi, A, Emara, M, Nicholls, J, Rossi, JJ & Habib, NA 2014, 'Exploiting human CD34+ stem cell-conditioned medium for tissue repair', Molecular Therapy, vol. 22, no. 1, pp. 149-159. https://doi.org/10.1038/mt.2013.194
Mintz PJ, Huang KW, Reebye V, Nteliopoulos G, Lai HS, Sætrom P et al. Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Molecular Therapy. 2014 Jan 1;22(1):149-159. https://doi.org/10.1038/mt.2013.194
Mintz, Paul J. ; Huang, Kai Wen ; Reebye, Vikash ; Nteliopoulos, Georgios ; Lai, Hong Shiee ; Sætrom, Pal ; Kasahara, Noriyuki ; Jensen, Steen ; Pai, Madhava ; Gordon, Myrtle Y A ; Marley, Stephen B. ; Behan, Rosemary ; Spalding, Duncan R. ; Haoudi, Abdelali ; Emara, Mohamed ; Nicholls, Joanna ; Rossi, John J. ; Habib, Nagy A. / Exploiting human CD34+ stem cell-conditioned medium for tissue repair. In: Molecular Therapy. 2014 ; Vol. 22, No. 1. pp. 149-159.
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