Exploiting human CD34+ stem cell-conditioned medium for tissue repair

Paul J. Mintz, Kai Wen Huang, Vikash Reebye, Georgios Nteliopoulos, Hong Shiee Lai, Pal Sætrom, Noriyuki Kasahara, Steen Jensen, Madhava Pai, Myrtle Y A Gordon, Stephen B. Marley, Rosemary Behan, Duncan R. Spalding, Abdelali Haoudi, Mohamed Emara, Joanna Nicholls, John J. Rossi, Nagy A. Habib

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6 Citations (Scopus)

Abstract

Despite the progress in our understanding of genes essential for stem cell regulation and development, little is known about the factors secreted by stem cells and their effect on tissue regeneration. In particular, the factors secreted by human CD34 + cells remain to be elucidated. We have approached this challenge by performing a cytokine/growth factor microarray analysis of secreted soluble factors in medium conditioned by adherent human CD34 + cells. Thirty-two abundantly secreted factors have been identified, all of which are associated with cell proliferation, survival, tissue repair, and wound healing. The cultured CD34 + cells expressed known stem cell genes such as Nanog, Oct4, Sox2, c-kit, and HoxB4. The conditioned medium containing the secreted factors prevented cell death in liver cells exposed to liver toxin in vitro via inhibition of the caspase-3 signaling pathway. More importantly, in vivo studies using animal models of liver damage demonstrated that injection of the conditioned medium could repair damaged liver tissue (significant reduction in the necroinflammatory activity), as well as enable the animals to survive. Thus, we demonstrate that medium conditioned by human CD34 + cells has the potential for therapeutic repair of damaged tissue in vivo.

Original languageEnglish
Pages (from-to)149-159
Number of pages11
JournalMolecular Therapy
Volume22
Issue number1
DOIs
Publication statusPublished - 1 Jan 2014

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ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Genetics
  • Drug Discovery
  • Pharmacology

Cite this

Mintz, P. J., Huang, K. W., Reebye, V., Nteliopoulos, G., Lai, H. S., Sætrom, P., Kasahara, N., Jensen, S., Pai, M., Gordon, M. Y. A., Marley, S. B., Behan, R., Spalding, D. R., Haoudi, A., Emara, M., Nicholls, J., Rossi, J. J., & Habib, N. A. (2014). Exploiting human CD34+ stem cell-conditioned medium for tissue repair. Molecular Therapy, 22(1), 149-159. https://doi.org/10.1038/mt.2013.194