Experimental polymyxin B-induced interstitial lung disease characterized by an accumulation of cytotoxic eosinophils in the alveolar structures

X. H. Sun, W. B. Davis, Y. Fukuda, V. J. Ferrans, Ronald Crystal

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A variety of lung disorders are associated with the accumulation of eosinophils in the alveolar structures. To help understand the role of eosinophils in these disorders, an animal model of eosinophilic lung disease was developed. Administration of an aerosol of polymyxin B to guinea pigs (3 times per wk for 4 wk) produced diffuse interstitial lung diseases with alveolar wall thickening and an alveolitis characterized by marked increases in eosinophilis and alveolar macrophages. Bronchoalveolar lavage confirmed the presence of significantly increased numbers of eosinophils and alveolar macrophages in polymyxin-B-treated animals compared with those in control animals. Using density gradient centrifugation, approximately 107 eosinophils could be purified from the lungs of a single polymyxin-B-treated animal. Importantly, eosinophils purified from the lungs from polymyxin B-treated animals exhibited significant spontaneous cellular cytotoxicity for human fetal lung fibroblasts. In contrast, neither eosinophils from control animals nor alveolar macrophages from either group of animals were cytotoxic. These findings demonstrate that eosinophils possess effector processes capable of injuring the lung parenchyma and suggest that eosinophils can contribute to the pathogenesis of the interstitial lung disease.

Original languageEnglish
Pages (from-to)103-108
Number of pages6
JournalAmerican Review of Respiratory Disease
Volume131
Issue number1
Publication statusPublished - 4 Apr 1985
Externally publishedYes

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Polymyxin B
Interstitial Lung Diseases
Eosinophils
Alveolar Macrophages
Lung
Density Gradient Centrifugation
Bronchoalveolar Lavage
Aerosols
Lung Diseases
Guinea Pigs
Animal Models
Fibroblasts

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Experimental polymyxin B-induced interstitial lung disease characterized by an accumulation of cytotoxic eosinophils in the alveolar structures. / Sun, X. H.; Davis, W. B.; Fukuda, Y.; Ferrans, V. J.; Crystal, Ronald.

In: American Review of Respiratory Disease, Vol. 131, No. 1, 04.04.1985, p. 103-108.

Research output: Contribution to journalArticle

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N2 - A variety of lung disorders are associated with the accumulation of eosinophils in the alveolar structures. To help understand the role of eosinophils in these disorders, an animal model of eosinophilic lung disease was developed. Administration of an aerosol of polymyxin B to guinea pigs (3 times per wk for 4 wk) produced diffuse interstitial lung diseases with alveolar wall thickening and an alveolitis characterized by marked increases in eosinophilis and alveolar macrophages. Bronchoalveolar lavage confirmed the presence of significantly increased numbers of eosinophils and alveolar macrophages in polymyxin-B-treated animals compared with those in control animals. Using density gradient centrifugation, approximately 107 eosinophils could be purified from the lungs of a single polymyxin-B-treated animal. Importantly, eosinophils purified from the lungs from polymyxin B-treated animals exhibited significant spontaneous cellular cytotoxicity for human fetal lung fibroblasts. In contrast, neither eosinophils from control animals nor alveolar macrophages from either group of animals were cytotoxic. These findings demonstrate that eosinophils possess effector processes capable of injuring the lung parenchyma and suggest that eosinophils can contribute to the pathogenesis of the interstitial lung disease.

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