Evolution of Metastases in Space and Time under Immune Selection

Mihaela Angelova, Bernhard Mlecnik, Angela Vasaturo, Gabriela Bindea, Tessa Fredriksen, Lucie Lafontaine, Bénédicte Buttard, Erwan Morgand, Daniela Bruni, Anne Jouret-Mourin, Catherine Hubert, Alex Kartheuser, Yves Humblet, Michele Ceccarelli, Najeeb Syed, Francesco M. Marincola, Davide Bedognetti, Marc Van den Eynde, Jérôme Galon

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56 Citations (Scopus)

Abstract

We examined how the immune microenvironment molds tumor evolution at different metastatic organs in a longitudinal dataset of colorectal cancer. Through multiplexed analyses, we showed that clonal evolution patterns during metastatic progression depend on the immune contexture at the metastatic site. Genetic evidence of neoantigen depletion was observed in the sites with high Immunoscore and spatial proximity between Ki67+ tumor cells and CD3+ cells. The immunoedited tumor clones were eliminated and did not recur, while progressing clones were immune privileged, despite the presence of tumor-infiltrating lymphocytes. Characterization of immune-privileged metastases revealed tumor-intrinsic and tumor-extrinsic mechanisms of escape. The lowest recurrence risk was associated with high Immunoscore, occurrence of immunoediting, and low tumor burden. We propose a parallel selection model of metastatic progression, where branched evolution could be traced back to immune-escaping clones. The findings could inform the understanding of cancer dissemination and the development of immunotherapeutics. A longitudinal analysis of clonal evolution of tumors across multiple tissues identifies a parallel selection model that explains the role of immune editing in controlling metastatic growth.

Original languageEnglish
Pages (from-to)751-765.e16
JournalCell
Volume175
Issue number3
DOIs
Publication statusPublished - 18 Oct 2018
Externally publishedYes

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Keywords

  • clonal
  • heterogeneity
  • immunoediting
  • immunoscore
  • immunotherapy
  • metastasis
  • microenvironment
  • mutations
  • recurrence
  • T cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Angelova, M., Mlecnik, B., Vasaturo, A., Bindea, G., Fredriksen, T., Lafontaine, L., Buttard, B., Morgand, E., Bruni, D., Jouret-Mourin, A., Hubert, C., Kartheuser, A., Humblet, Y., Ceccarelli, M., Syed, N., Marincola, F. M., Bedognetti, D., Van den Eynde, M., & Galon, J. (2018). Evolution of Metastases in Space and Time under Immune Selection. Cell, 175(3), 751-765.e16. https://doi.org/10.1016/j.cell.2018.09.018