Evidence for P-glycoprotein involvement in cell volume regulation using coulter sizing in flow cytometry

Jennifer Pasquier, Damien Rioult, Nadine Abu-Kaoud, Jessica Hoarau, Matthieu Marin, Frank Le Foll

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The regulation of cell volume is an essential function that is coupled to a variety of physiological processes such as receptor recycling, excitability and contraction, cell proliferation, migration, and programmed cell death. Under stress, cells undergo emergency swelling and respond to such a phenomenon with a regulatory volume decrease (RVD) where they release cellular ions, and other osmolytes as well as a concomitant loss of water. The link between P-glycoprotein, a transmembrane transporter, and cell volume regulation is controversial, and changes in cells volume are measured using microscopy or electrophysiology. For instance, by using the patch-clamp method, our team demonstrated that chloride currents activated in the RVD were more intense and rapid in a breast cancer cell line overexpressing the P-glycoprotein (P-gp). The Cell Lab Quanta SC is a flow cytometry system that simultaneously measures electronic volume, side scatter and three fluorescent colors; altogether this provides unsurpassed population resolution and accurate cell counting. Therefore, here we propose a novel method to follow cellular volume. By using the Coulter-type channel of the cytometer Cell Lab Quanta SC MPL (multi-platform loading), we demonstrated a role for the P-gp during different osmotic treatments, but also a differential activity of the P-gp through the cell cycle. Altogether, our data strongly suggests a role of P-gp in cell volume regulation.

Original languageEnglish
Pages (from-to)14318-14337
Number of pages20
JournalInternational Journal of Molecular Sciences
Volume16
Issue number7
DOIs
Publication statusPublished - 24 Jun 2015

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Keywords

  • Cell volume
  • Flow cytometer
  • MCF7
  • Multi-drug resistance
  • P-glycoprotein

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Spectroscopy
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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