Evidence for a shared structural role for HMG1 and linker histones B4 and H1 in organizing chromatin

Karl Nightingale, Stefan Dimitrov, Raymond Reeves, Alan P. Wolffe

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

The high mobility group proteins 1 and 2 (HMG1/2) and histone B4 are major components of chromatin within the nuclei assembled during the incubation of Xenopus sperm chromatin in Xenopus egg extract. To investigate their potential structural and functional roles, we have cloned and expressed Xenopus HMG1 and histone B4. Purified histone B4 and HMG1 form stable complexes with nucleosomes including Xenopus 5S DNA. Both proteins associate with linker DNA and stabilize it against digestion with micrococcal nuclease, in a similar manner to histone H1. However, neither histone B4 nor HMG1 influence the DNase I or hydroxyl radical digestion of DNA within the nucleosome core. We suggest that HMG1/2 and histone B4 have a shared structural role in organizing linker DNA in the nucleosome.

Original languageEnglish
Pages (from-to)548-561
Number of pages14
JournalEMBO Journal
Volume15
Issue number3
Publication statusPublished - 1 Feb 1996

    Fingerprint

Keywords

  • B4/HMG1/nucleosome/Xenopus
  • Chromatin/histone

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this