Evaluation of various cell drying and disruption techniques for sustainable metabolite extractions from microalgae grown in wastewater

A multivariate approach

Faiz Ahmad Ansari, Sanjay Kumar Gupta, Mahmoud Nasr, Ismail Rawat, Faizal Bux

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This study attempted to determine the yield of extractable metabolites and the cost of oil production from Scenedesmus obliquus cultivated in municipal wastewater. The microalgae achieved a biomass concentration of 1.64 g L−1 as well as pollutant removal efficiencies of 87.86% COD, 86.16% NH4 +-N, and ≈100% PO4 3−-P. The harvested microalgae were subjected to different drying and cell disruption techniques for lipid extraction, and the residual biomass was used to recover proteins and carbohydrates. Principal component analysis was employed to evaluate the metabolic yields obtained from sun-, freeze-, and oven-drying methods and microwave-, sonication-, autoclaving-, and osmotic shock-disruption techniques. The lipid yield varied between 4.90± 0.42% for sun-dried biomass subjected to osmotic shock and 25.39± 1.08% for freeze-drying with microwave-assisted extraction. Protein yield of the whole microalgae cells (31.26± 3.76%) was comparable (p > 0.05) to that resulting from lipid-extracted microalgae by either autoclaving or osmotic shock. Carbohydrate yield of the intact microalgae cells (19.80± 1.49%) was comparable (p > 0.05) to that of lipid-extracted microalgae from amongst all the cell disruption methods. Results of a techno-economic analysis indicated that the cost of oil production from microalgae varied between $0.883 and $2.088 per liter. These results revealed the feasibility of using a sequential extraction of lipids followed by proteins and carbohydrates.

Original languageEnglish
Pages (from-to)634-643
Number of pages10
JournalJournal of Cleaner Production
Volume182
DOIs
Publication statusPublished - 1 May 2018

Fingerprint

Metabolites
Lipids
metabolite
Drying
Wastewater
lipid
wastewater
Carbohydrates
carbohydrate
Biomass
Proteins
Sun
oil production
protein
biomass
Microwaves
freeze drying
pollutant removal
Sonication
Economic analysis

Keywords

  • Drying cell disruption
  • Metabolites extraction
  • Multivariate analysis
  • Oil production cost
  • Scenedesmus obliquus

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)
  • Strategy and Management
  • Industrial and Manufacturing Engineering

Cite this

Evaluation of various cell drying and disruption techniques for sustainable metabolite extractions from microalgae grown in wastewater : A multivariate approach. / Ansari, Faiz Ahmad; Gupta, Sanjay Kumar; Nasr, Mahmoud; Rawat, Ismail; Bux, Faizal.

In: Journal of Cleaner Production, Vol. 182, 01.05.2018, p. 634-643.

Research output: Contribution to journalArticle

@article{6389965fc40f487aaae0053bc716b891,
title = "Evaluation of various cell drying and disruption techniques for sustainable metabolite extractions from microalgae grown in wastewater: A multivariate approach",
abstract = "This study attempted to determine the yield of extractable metabolites and the cost of oil production from Scenedesmus obliquus cultivated in municipal wastewater. The microalgae achieved a biomass concentration of 1.64 g L−1 as well as pollutant removal efficiencies of 87.86{\%} COD, 86.16{\%} NH4 +-N, and ≈100{\%} PO4 3−-P. The harvested microalgae were subjected to different drying and cell disruption techniques for lipid extraction, and the residual biomass was used to recover proteins and carbohydrates. Principal component analysis was employed to evaluate the metabolic yields obtained from sun-, freeze-, and oven-drying methods and microwave-, sonication-, autoclaving-, and osmotic shock-disruption techniques. The lipid yield varied between 4.90± 0.42{\%} for sun-dried biomass subjected to osmotic shock and 25.39± 1.08{\%} for freeze-drying with microwave-assisted extraction. Protein yield of the whole microalgae cells (31.26± 3.76{\%}) was comparable (p > 0.05) to that resulting from lipid-extracted microalgae by either autoclaving or osmotic shock. Carbohydrate yield of the intact microalgae cells (19.80± 1.49{\%}) was comparable (p > 0.05) to that of lipid-extracted microalgae from amongst all the cell disruption methods. Results of a techno-economic analysis indicated that the cost of oil production from microalgae varied between $0.883 and $2.088 per liter. These results revealed the feasibility of using a sequential extraction of lipids followed by proteins and carbohydrates.",
keywords = "Drying cell disruption, Metabolites extraction, Multivariate analysis, Oil production cost, Scenedesmus obliquus",
author = "Ansari, {Faiz Ahmad} and Gupta, {Sanjay Kumar} and Mahmoud Nasr and Ismail Rawat and Faizal Bux",
year = "2018",
month = "5",
day = "1",
doi = "10.1016/j.jclepro.2018.02.098",
language = "English",
volume = "182",
pages = "634--643",
journal = "Journal of Cleaner Production",
issn = "0959-6526",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Evaluation of various cell drying and disruption techniques for sustainable metabolite extractions from microalgae grown in wastewater

T2 - A multivariate approach

AU - Ansari, Faiz Ahmad

AU - Gupta, Sanjay Kumar

AU - Nasr, Mahmoud

AU - Rawat, Ismail

AU - Bux, Faizal

PY - 2018/5/1

Y1 - 2018/5/1

N2 - This study attempted to determine the yield of extractable metabolites and the cost of oil production from Scenedesmus obliquus cultivated in municipal wastewater. The microalgae achieved a biomass concentration of 1.64 g L−1 as well as pollutant removal efficiencies of 87.86% COD, 86.16% NH4 +-N, and ≈100% PO4 3−-P. The harvested microalgae were subjected to different drying and cell disruption techniques for lipid extraction, and the residual biomass was used to recover proteins and carbohydrates. Principal component analysis was employed to evaluate the metabolic yields obtained from sun-, freeze-, and oven-drying methods and microwave-, sonication-, autoclaving-, and osmotic shock-disruption techniques. The lipid yield varied between 4.90± 0.42% for sun-dried biomass subjected to osmotic shock and 25.39± 1.08% for freeze-drying with microwave-assisted extraction. Protein yield of the whole microalgae cells (31.26± 3.76%) was comparable (p > 0.05) to that resulting from lipid-extracted microalgae by either autoclaving or osmotic shock. Carbohydrate yield of the intact microalgae cells (19.80± 1.49%) was comparable (p > 0.05) to that of lipid-extracted microalgae from amongst all the cell disruption methods. Results of a techno-economic analysis indicated that the cost of oil production from microalgae varied between $0.883 and $2.088 per liter. These results revealed the feasibility of using a sequential extraction of lipids followed by proteins and carbohydrates.

AB - This study attempted to determine the yield of extractable metabolites and the cost of oil production from Scenedesmus obliquus cultivated in municipal wastewater. The microalgae achieved a biomass concentration of 1.64 g L−1 as well as pollutant removal efficiencies of 87.86% COD, 86.16% NH4 +-N, and ≈100% PO4 3−-P. The harvested microalgae were subjected to different drying and cell disruption techniques for lipid extraction, and the residual biomass was used to recover proteins and carbohydrates. Principal component analysis was employed to evaluate the metabolic yields obtained from sun-, freeze-, and oven-drying methods and microwave-, sonication-, autoclaving-, and osmotic shock-disruption techniques. The lipid yield varied between 4.90± 0.42% for sun-dried biomass subjected to osmotic shock and 25.39± 1.08% for freeze-drying with microwave-assisted extraction. Protein yield of the whole microalgae cells (31.26± 3.76%) was comparable (p > 0.05) to that resulting from lipid-extracted microalgae by either autoclaving or osmotic shock. Carbohydrate yield of the intact microalgae cells (19.80± 1.49%) was comparable (p > 0.05) to that of lipid-extracted microalgae from amongst all the cell disruption methods. Results of a techno-economic analysis indicated that the cost of oil production from microalgae varied between $0.883 and $2.088 per liter. These results revealed the feasibility of using a sequential extraction of lipids followed by proteins and carbohydrates.

KW - Drying cell disruption

KW - Metabolites extraction

KW - Multivariate analysis

KW - Oil production cost

KW - Scenedesmus obliquus

UR - http://www.scopus.com/inward/record.url?scp=85043601667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043601667&partnerID=8YFLogxK

U2 - 10.1016/j.jclepro.2018.02.098

DO - 10.1016/j.jclepro.2018.02.098

M3 - Article

VL - 182

SP - 634

EP - 643

JO - Journal of Cleaner Production

JF - Journal of Cleaner Production

SN - 0959-6526

ER -