Evaluation of the maturation of individual Dengue virions with flow virometry

Sonia Zicari, Anush Arakelyan, Wendy Fitzgerald, Elena Zaitseva, Leonid V. Chernomordik, Leonid Margolis, Jean-Charles B. Grivel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

High-throughput techniques are needed to analyze individual virions to understand how viral heterogeneity translates into pathogenesis since in bulk analysis the individual characteristics of virions are lost. Individual Dengue virions (DENV) undergo a maturation that involves a proteolytic cleavage of prM precursor into virion-associated M protein. Here, using a new nanoparticle-based technology, "flow virometry", we compared the maturation of individual DENV produced by BHK-21 and LoVo cells. The latter lacks the furin-protease that mediates prM cleavage. We found that prM is present on about 50% of DENV particles produced in BHK-21 cells and about 85% of DENV virions produced in LoVo, indicating an increase in the fraction of not fully matured virions. Flow virometry allows us to quantify the number of fully mature particles in DENV preparations and proves to be a useful method for studying heterogeneity of the surface proteins of various viruses.

Original languageEnglish
Pages (from-to)20-27
Number of pages8
JournalVirology
Volume488
DOIs
Publication statusPublished - 15 Jan 2016

    Fingerprint

Keywords

  • Dengue
  • Flow virometry
  • Maturation
  • PrM

ASJC Scopus subject areas

  • Virology

Cite this

Zicari, S., Arakelyan, A., Fitzgerald, W., Zaitseva, E., Chernomordik, L. V., Margolis, L., & Grivel, J-C. B. (2016). Evaluation of the maturation of individual Dengue virions with flow virometry. Virology, 488, 20-27. https://doi.org/10.1016/j.virol.2015.10.021