Evaluation of contact lens-induced changes in keratoconic corneas using in vivo confocal microscopy

Gulfidan Bitirgen, Ahmet Ozkagnici, Rayaz Malik, Refik Oltulu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

PURPOSE. To quantitatively analyze laser scanning in vivo confocal microscopy (IVCM) images of all corneal layers in contact lens-wearing and noncontact lens-wearing keratoconus patients. METHODS. The study population included rigid gas permeable (RGP) contact lens-wearing keratoconus patients (group 1; N = 29), keratoconus patients who did not wear contact lenses (group 2; N = 30), and subjects who neither had keratoconus nor wore contact lenses (group 3; N = 30), with groups 2 and 3 matched to group 1 by age and sex. The central cornea was examined with IVCM in all subjects. The mean duration of contact lens wear was 5.50 ± 3.68 years (range, 2-15 years). RESULTS. Eyes with keratoconus showed significantly lower basal epithelial cell and anterior and posterior stromal keratocyte densities, as well as subbasal nerve fiber density, nerve branch density, and nerve fiber length compared with healthy control subjects. Furthermore, compared with group 2, group 1 had significantly lower basal epithelial cell density (4920 ± 476 cells/mm2 vs. 4503 ± 461 cells/mm2, P = 0.001) and anterior stromal keratocyte density (561 ± 91 cells/mm2 vs. 464 ± 55 cells/mm2, P < 0.001), but there was no significant difference for posterior stromal keratocyte density (P = 0.808), endothelial cell density (P = 0.699), or subbasal nerve fiber density (P = 0.142), nerve branch density (P = 0.614), and nerve fiber length (P = 0.850). CONCLUSIONS. Significant corneal microstructural abnormalities were observed in eyes with keratoconus. RGP contact lens wear was associated with a further reduction in the basal epithelial cell and anterior stromal keratocyte densities, but with no effect on posterior stromal keratocyte density, endothelial cell density, or corneal nerve morphology.

Original languageEnglish
Pages (from-to)5385-5391
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume54
Issue number8
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Keratoconus
Contact Lenses
Confocal Microscopy
Cornea
Nerve Fibers
Cell Count
Epithelial Cells
Endothelial Cells
Gases
Lenses
Intravital Microscopy
Healthy Volunteers
Lasers
Research Design
Population

Keywords

  • Confocal microscopy
  • Contact lens
  • Cornea
  • Keratoconus

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Evaluation of contact lens-induced changes in keratoconic corneas using in vivo confocal microscopy. / Bitirgen, Gulfidan; Ozkagnici, Ahmet; Malik, Rayaz; Oltulu, Refik.

In: Investigative Ophthalmology and Visual Science, Vol. 54, No. 8, 2013, p. 5385-5391.

Research output: Contribution to journalArticle

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T1 - Evaluation of contact lens-induced changes in keratoconic corneas using in vivo confocal microscopy

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AU - Malik, Rayaz

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PY - 2013

Y1 - 2013

N2 - PURPOSE. To quantitatively analyze laser scanning in vivo confocal microscopy (IVCM) images of all corneal layers in contact lens-wearing and noncontact lens-wearing keratoconus patients. METHODS. The study population included rigid gas permeable (RGP) contact lens-wearing keratoconus patients (group 1; N = 29), keratoconus patients who did not wear contact lenses (group 2; N = 30), and subjects who neither had keratoconus nor wore contact lenses (group 3; N = 30), with groups 2 and 3 matched to group 1 by age and sex. The central cornea was examined with IVCM in all subjects. The mean duration of contact lens wear was 5.50 ± 3.68 years (range, 2-15 years). RESULTS. Eyes with keratoconus showed significantly lower basal epithelial cell and anterior and posterior stromal keratocyte densities, as well as subbasal nerve fiber density, nerve branch density, and nerve fiber length compared with healthy control subjects. Furthermore, compared with group 2, group 1 had significantly lower basal epithelial cell density (4920 ± 476 cells/mm2 vs. 4503 ± 461 cells/mm2, P = 0.001) and anterior stromal keratocyte density (561 ± 91 cells/mm2 vs. 464 ± 55 cells/mm2, P < 0.001), but there was no significant difference for posterior stromal keratocyte density (P = 0.808), endothelial cell density (P = 0.699), or subbasal nerve fiber density (P = 0.142), nerve branch density (P = 0.614), and nerve fiber length (P = 0.850). CONCLUSIONS. Significant corneal microstructural abnormalities were observed in eyes with keratoconus. RGP contact lens wear was associated with a further reduction in the basal epithelial cell and anterior stromal keratocyte densities, but with no effect on posterior stromal keratocyte density, endothelial cell density, or corneal nerve morphology.

AB - PURPOSE. To quantitatively analyze laser scanning in vivo confocal microscopy (IVCM) images of all corneal layers in contact lens-wearing and noncontact lens-wearing keratoconus patients. METHODS. The study population included rigid gas permeable (RGP) contact lens-wearing keratoconus patients (group 1; N = 29), keratoconus patients who did not wear contact lenses (group 2; N = 30), and subjects who neither had keratoconus nor wore contact lenses (group 3; N = 30), with groups 2 and 3 matched to group 1 by age and sex. The central cornea was examined with IVCM in all subjects. The mean duration of contact lens wear was 5.50 ± 3.68 years (range, 2-15 years). RESULTS. Eyes with keratoconus showed significantly lower basal epithelial cell and anterior and posterior stromal keratocyte densities, as well as subbasal nerve fiber density, nerve branch density, and nerve fiber length compared with healthy control subjects. Furthermore, compared with group 2, group 1 had significantly lower basal epithelial cell density (4920 ± 476 cells/mm2 vs. 4503 ± 461 cells/mm2, P = 0.001) and anterior stromal keratocyte density (561 ± 91 cells/mm2 vs. 464 ± 55 cells/mm2, P < 0.001), but there was no significant difference for posterior stromal keratocyte density (P = 0.808), endothelial cell density (P = 0.699), or subbasal nerve fiber density (P = 0.142), nerve branch density (P = 0.614), and nerve fiber length (P = 0.850). CONCLUSIONS. Significant corneal microstructural abnormalities were observed in eyes with keratoconus. RGP contact lens wear was associated with a further reduction in the basal epithelial cell and anterior stromal keratocyte densities, but with no effect on posterior stromal keratocyte density, endothelial cell density, or corneal nerve morphology.

KW - Confocal microscopy

KW - Contact lens

KW - Cornea

KW - Keratoconus

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