Equal force recovery in dysferlin-deficient and wild-type muscles following saponin exposure

Piming Zhao, Li Xu, Younss Ait-Mou, Pieter P. De Tombe, Renzhi Han

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Dysferlin plays an important role in repairing membrane damage elicited by laser irradiation, and dysferlin deficiency causes muscular dystrophy and associated cardiomyopathy. Proteins such as perforin, complement component C9, and bacteria-derived cytolysins, as well as the natural detergent saponin, can form large pores on the cell membrane via complexation with cholesterol. However, it is not clear whether dysferlin plays a role in repairing membrane damage induced by pore-forming reagents. In this study, we observed that dysferlin-deficient muscles recovered the tetanic force production to the same extent as their WT counterparts following a 5-min saponin exposure (50 μg/mL). Interestingly, the slow soleus muscles recovered significantly better than the fast extensor digitorum longus (EDL) muscles. Our data suggest that dysferlin is unlikely involved in repairing saponin-induced membrane damage and that the slow muscle is more efficient than the fast muscle in repairing such damage.

Original languageEnglish
Article number235216
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Saponins
Muscle
Recovery
Muscles
Membranes
Complement C9
Perforin
Muscular Dystrophies
Cytotoxins
Cardiomyopathies
Detergents
Laser beam effects
Cell membranes
Skeletal Muscle
Lasers
Complexation
Cholesterol
Cell Membrane
Bacteria
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Genetics
  • Molecular Biology
  • Health, Toxicology and Mutagenesis
  • Medicine(all)

Cite this

Equal force recovery in dysferlin-deficient and wild-type muscles following saponin exposure. / Zhao, Piming; Xu, Li; Ait-Mou, Younss; De Tombe, Pieter P.; Han, Renzhi.

In: Journal of Biomedicine and Biotechnology, Vol. 2011, 235216, 2011.

Research output: Contribution to journalArticle

Zhao, Piming ; Xu, Li ; Ait-Mou, Younss ; De Tombe, Pieter P. ; Han, Renzhi. / Equal force recovery in dysferlin-deficient and wild-type muscles following saponin exposure. In: Journal of Biomedicine and Biotechnology. 2011 ; Vol. 2011.
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