Epitopes expressed in different adenoviras capsid proteins induce different levels of epitope-specific immunity

Anja Krause, Ju H. Joh, Neil R. Hackett, Peter W. Roelvink, Joseph T. Bruder, Thomas J. Wickham, Imre Kovesdi, Ronald Crystal, Stefan Worgall

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

On the basis of the concept that the capsid proteins of adenovinis (Ad) gene transfer vectors can be genetically manipulated to enhance the immunogenicity of Ad-based vaccines, the present study compared the antiantigen immunogenicity of Ad vectors with a common epitope of the hemagglutinin (HA) protein of the influenza A virus incorporated into the outer Ad capsid protein hexon, penton base, fiber knob, or protein IX. Incorporation of the same epitope into the different capsid proteins provided insights into the correlation between epitope position and antiepitope immunity. Following immunization of three different strains of mice (C57BL/6, BALB/c, and CBA) with either an equal number of Ad particles (resulting in a different total HA copy number) or different Ad particle numbers (to achieve the same HA copy number), the highest primary (immunoglobulin M [IgM]) and secondary (IgG) anti-HA humoral and cellular CD4 gamma interferon and interleukin-4 responses against HA were always achieved with the Ad vector carrying the HA epitope in fiber knob. These observations suggest that the immune response against an epitope inserted into Ad capsid proteins is not necessarily dependent on the capsid protein number and imply that the choice of incorporation site in Ad capsid proteins in their use as vaccines needs to be compared in vivo.

Original languageEnglish
Pages (from-to)5523-5530
Number of pages8
JournalJournal of Virology
Volume80
Issue number11
DOIs
Publication statusPublished - 1 Jun 2006
Externally publishedYes

Fingerprint

Hemagglutinins
Capsid Proteins
Epitopes
Immunity
Vaccines
Influenza A virus
Inbred C57BL Mouse
Interleukin-4
Interferon-gamma
Immunoglobulin M
Immunization
Proteins
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Krause, A., Joh, J. H., Hackett, N. R., Roelvink, P. W., Bruder, J. T., Wickham, T. J., ... Worgall, S. (2006). Epitopes expressed in different adenoviras capsid proteins induce different levels of epitope-specific immunity. Journal of Virology, 80(11), 5523-5530. https://doi.org/10.1128/JVI.02667-05

Epitopes expressed in different adenoviras capsid proteins induce different levels of epitope-specific immunity. / Krause, Anja; Joh, Ju H.; Hackett, Neil R.; Roelvink, Peter W.; Bruder, Joseph T.; Wickham, Thomas J.; Kovesdi, Imre; Crystal, Ronald; Worgall, Stefan.

In: Journal of Virology, Vol. 80, No. 11, 01.06.2006, p. 5523-5530.

Research output: Contribution to journalArticle

Krause, A, Joh, JH, Hackett, NR, Roelvink, PW, Bruder, JT, Wickham, TJ, Kovesdi, I, Crystal, R & Worgall, S 2006, 'Epitopes expressed in different adenoviras capsid proteins induce different levels of epitope-specific immunity', Journal of Virology, vol. 80, no. 11, pp. 5523-5530. https://doi.org/10.1128/JVI.02667-05
Krause, Anja ; Joh, Ju H. ; Hackett, Neil R. ; Roelvink, Peter W. ; Bruder, Joseph T. ; Wickham, Thomas J. ; Kovesdi, Imre ; Crystal, Ronald ; Worgall, Stefan. / Epitopes expressed in different adenoviras capsid proteins induce different levels of epitope-specific immunity. In: Journal of Virology. 2006 ; Vol. 80, No. 11. pp. 5523-5530.
@article{64fb7c62cb2e47a683ccf9ab3494259c,
title = "Epitopes expressed in different adenoviras capsid proteins induce different levels of epitope-specific immunity",
abstract = "On the basis of the concept that the capsid proteins of adenovinis (Ad) gene transfer vectors can be genetically manipulated to enhance the immunogenicity of Ad-based vaccines, the present study compared the antiantigen immunogenicity of Ad vectors with a common epitope of the hemagglutinin (HA) protein of the influenza A virus incorporated into the outer Ad capsid protein hexon, penton base, fiber knob, or protein IX. Incorporation of the same epitope into the different capsid proteins provided insights into the correlation between epitope position and antiepitope immunity. Following immunization of three different strains of mice (C57BL/6, BALB/c, and CBA) with either an equal number of Ad particles (resulting in a different total HA copy number) or different Ad particle numbers (to achieve the same HA copy number), the highest primary (immunoglobulin M [IgM]) and secondary (IgG) anti-HA humoral and cellular CD4 gamma interferon and interleukin-4 responses against HA were always achieved with the Ad vector carrying the HA epitope in fiber knob. These observations suggest that the immune response against an epitope inserted into Ad capsid proteins is not necessarily dependent on the capsid protein number and imply that the choice of incorporation site in Ad capsid proteins in their use as vaccines needs to be compared in vivo.",
author = "Anja Krause and Joh, {Ju H.} and Hackett, {Neil R.} and Roelvink, {Peter W.} and Bruder, {Joseph T.} and Wickham, {Thomas J.} and Imre Kovesdi and Ronald Crystal and Stefan Worgall",
year = "2006",
month = "6",
day = "1",
doi = "10.1128/JVI.02667-05",
language = "English",
volume = "80",
pages = "5523--5530",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Epitopes expressed in different adenoviras capsid proteins induce different levels of epitope-specific immunity

AU - Krause, Anja

AU - Joh, Ju H.

AU - Hackett, Neil R.

AU - Roelvink, Peter W.

AU - Bruder, Joseph T.

AU - Wickham, Thomas J.

AU - Kovesdi, Imre

AU - Crystal, Ronald

AU - Worgall, Stefan

PY - 2006/6/1

Y1 - 2006/6/1

N2 - On the basis of the concept that the capsid proteins of adenovinis (Ad) gene transfer vectors can be genetically manipulated to enhance the immunogenicity of Ad-based vaccines, the present study compared the antiantigen immunogenicity of Ad vectors with a common epitope of the hemagglutinin (HA) protein of the influenza A virus incorporated into the outer Ad capsid protein hexon, penton base, fiber knob, or protein IX. Incorporation of the same epitope into the different capsid proteins provided insights into the correlation between epitope position and antiepitope immunity. Following immunization of three different strains of mice (C57BL/6, BALB/c, and CBA) with either an equal number of Ad particles (resulting in a different total HA copy number) or different Ad particle numbers (to achieve the same HA copy number), the highest primary (immunoglobulin M [IgM]) and secondary (IgG) anti-HA humoral and cellular CD4 gamma interferon and interleukin-4 responses against HA were always achieved with the Ad vector carrying the HA epitope in fiber knob. These observations suggest that the immune response against an epitope inserted into Ad capsid proteins is not necessarily dependent on the capsid protein number and imply that the choice of incorporation site in Ad capsid proteins in their use as vaccines needs to be compared in vivo.

AB - On the basis of the concept that the capsid proteins of adenovinis (Ad) gene transfer vectors can be genetically manipulated to enhance the immunogenicity of Ad-based vaccines, the present study compared the antiantigen immunogenicity of Ad vectors with a common epitope of the hemagglutinin (HA) protein of the influenza A virus incorporated into the outer Ad capsid protein hexon, penton base, fiber knob, or protein IX. Incorporation of the same epitope into the different capsid proteins provided insights into the correlation between epitope position and antiepitope immunity. Following immunization of three different strains of mice (C57BL/6, BALB/c, and CBA) with either an equal number of Ad particles (resulting in a different total HA copy number) or different Ad particle numbers (to achieve the same HA copy number), the highest primary (immunoglobulin M [IgM]) and secondary (IgG) anti-HA humoral and cellular CD4 gamma interferon and interleukin-4 responses against HA were always achieved with the Ad vector carrying the HA epitope in fiber knob. These observations suggest that the immune response against an epitope inserted into Ad capsid proteins is not necessarily dependent on the capsid protein number and imply that the choice of incorporation site in Ad capsid proteins in their use as vaccines needs to be compared in vivo.

UR - http://www.scopus.com/inward/record.url?scp=33646742581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646742581&partnerID=8YFLogxK

U2 - 10.1128/JVI.02667-05

DO - 10.1128/JVI.02667-05

M3 - Article

C2 - 16699033

AN - SCOPUS:33646742581

VL - 80

SP - 5523

EP - 5530

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 11

ER -