Enzyme linked immunoassay (elisa) for connective tissue proteins: Type 1 collagen

Stephen I. Rennard, George R. Martin, Ronald Crystal

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The classical approaches for the quantitation of collagen take advantage of unique features of collagen such as the presence of hydroxyproline 1 or the sensitivity of the protein to bacterial collagenase. 2 However, since these features are common to the different molecular species of collagen, 3 other methods are needed to selectively measure each collagen type. One approach to this problem is to capitalize on the fact that the different collagen types are immunologically distinct and antibodies with high binding affinity specific for each collagen type can be produced.4 Such antibodies can be utilized to quantify human type I collagen in an enzyme-linked immunoassay (ELISA)5 .6 which is sensitive, specific and easily performed. This assay offers several practical advantages over the more familiar radioimmunoassays.7.8 There are two basic types of ELISA tests, direct and inhibition. Both utilize the sensitivity of an enzyme reaction to measure the antigen antibody interaction. Since very sensitive enzyme-substrate-product combinations are available, the test is very powerful.

Original languageEnglish
Title of host publicationImmunochemistry of the Extracellular Matrix
Subtitle of host publicationVolume 1: Methods
PublisherCRC Press
Pages237-252
Number of pages16
ISBN (Electronic)9781351081863
ISBN (Print)0849361966, 9781315894317
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Collagen Type I
Immunoenzyme Techniques
Connective Tissue
Collagen
Tissue
Enzymes
Proteins
Antibodies
Bacterial Proteins
Hydroxyproline
Collagenases
Assays
Antigens
Substrates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Rennard, S. I., Martin, G. R., & Crystal, R. (2018). Enzyme linked immunoassay (elisa) for connective tissue proteins: Type 1 collagen. In Immunochemistry of the Extracellular Matrix: Volume 1: Methods (pp. 237-252). CRC Press. https://doi.org/10.1201/9781351073417

Enzyme linked immunoassay (elisa) for connective tissue proteins : Type 1 collagen. / Rennard, Stephen I.; Martin, George R.; Crystal, Ronald.

Immunochemistry of the Extracellular Matrix: Volume 1: Methods. CRC Press, 2018. p. 237-252.

Research output: Chapter in Book/Report/Conference proceedingChapter

Rennard, SI, Martin, GR & Crystal, R 2018, Enzyme linked immunoassay (elisa) for connective tissue proteins: Type 1 collagen. in Immunochemistry of the Extracellular Matrix: Volume 1: Methods. CRC Press, pp. 237-252. https://doi.org/10.1201/9781351073417
Rennard SI, Martin GR, Crystal R. Enzyme linked immunoassay (elisa) for connective tissue proteins: Type 1 collagen. In Immunochemistry of the Extracellular Matrix: Volume 1: Methods. CRC Press. 2018. p. 237-252 https://doi.org/10.1201/9781351073417
Rennard, Stephen I. ; Martin, George R. ; Crystal, Ronald. / Enzyme linked immunoassay (elisa) for connective tissue proteins : Type 1 collagen. Immunochemistry of the Extracellular Matrix: Volume 1: Methods. CRC Press, 2018. pp. 237-252
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