Enzymatic biodiesel production of microalgae lipids under supercritical carbon dioxide: Process optimization and integration

Hanifa Taher, Sulaiman Al-Zuhair, Ali H. Al-Marzouqi, Yousef Haik, Mohammed Farid

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Enzymatic biodiesel production in supercritical CO2 (SC-CO2) has recently received an increasing attention, as an alternative to the conventional chemical processes. In this study, enzymatic production of biodiesel from microalgal lipids was investigated in batch and integrated extraction-reaction systems. In the batch system, the effect of enzyme loading (15-50wt%), temperature (35-55°C) and methanol to lipid molar ratios (3-15:1) were studied, and response surface methodology was employed to optimize selected factors effect. The optimum transesterification yield of 80% was obtained at 47°C, 200bar, 35% enzyme loading, and 9:1 molar ratio after 4h reaction in the batch system. The experimental results were also used to determine the kinetics parameters of the Ping-Pong Bi Bi model, with methanol inhibition, suggested to describe the reaction. In the continuous integrated extraction-reaction system, the effect of methanol to lipids molar ratio was investigated, and enzyme operational stability and reusability were tested. Bed regeneration by tert-butanol washing was also assessed. The optimum methanol to lipid ratio was found to be 10:1. At this ratio, the enzyme was able to attain 78% of its original activity when reused for 6 continuous cycles, and the bed was successfully reused by washing with tert-butanol.

Original languageEnglish
Pages (from-to)103-113
Number of pages11
JournalBiochemical Engineering Journal
Volume90
DOIs
Publication statusPublished - 15 Sep 2014
Externally publishedYes

Fingerprint

Carbon dioxide process
Microalgae
Biofuels
Biodiesel
Carbon Dioxide
Lipids
Methanol
Enzymes
tert-Butyl Alcohol
Butenes
Washing
Chemical Phenomena
Enzyme Stability
Transesterification
Reusability
Kinetic parameters
Regeneration
Temperature

Keywords

  • Biodiesel
  • Lipase
  • Microalgae lipid
  • Process integration
  • Production kinetics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Environmental Engineering

Cite this

Enzymatic biodiesel production of microalgae lipids under supercritical carbon dioxide : Process optimization and integration. / Taher, Hanifa; Al-Zuhair, Sulaiman; Al-Marzouqi, Ali H.; Haik, Yousef; Farid, Mohammed.

In: Biochemical Engineering Journal, Vol. 90, 15.09.2014, p. 103-113.

Research output: Contribution to journalArticle

Taher, Hanifa ; Al-Zuhair, Sulaiman ; Al-Marzouqi, Ali H. ; Haik, Yousef ; Farid, Mohammed. / Enzymatic biodiesel production of microalgae lipids under supercritical carbon dioxide : Process optimization and integration. In: Biochemical Engineering Journal. 2014 ; Vol. 90. pp. 103-113.
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