Enhancement of osteoblast gene expression by mechanically compatible porous Si-rich nanocomposite

Gautam Gupta, Ahmed El-Ghannam, Sreenatha Kirakodu, Marwan Khraisheh, Hussein Zbib

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Synthesis of a porous bioactive ceramic implant for load bearing applications is a challenging task in maxillofacial and orthopedic surgeries. A novel bioactive resorbable silica-calcium phosphate nanocomposite (SCPC) has recently been introduced as a potential bone graft. In the present study, we employed SCPC to develop a resorbable porous scaffold and analyzed the effects of composition and porosity on the mechanical properties. The ranges of compressive strength and modulus of elasticity of SCPC containing 32-56% porosity were 1.5-50 MPa and 0.14-2.1 GPa, respectively, which matched the corresponding values for trabecular bone. The compressive strength of dense SCPC was dependent on the Si content and acquired values (93-285 MPa) comparable to that of cortical bone. The superior mechanical properties of SCPC are attributed to the intricate interactions at the boundaries of the nanograins and to the homogenous distribution of hierarchical pore-structure throughout the material volume. X-ray computed tomography and mercury porosimetry analyses revealed high interconnectivity of the pores in the size range 3 nm to 650 μm. Quantitative real-time PCR analyses showed that neonatal rat calvarial osteoblasts attached to Si-rich SCPC expressed 5- and 26-fold higher osteocalcin mRNA levels compared to cells attached to ProOsteon™ hydroxyapatite disks and tissue culture polystyrene plates respectively, after four days in culture. Results of the present study strongly suggest that porous, bioactive resorbable SCPCs can serve as tissue engineering scaffolds for cell delivery to treat load-bearing bone defects in orthopedic and maxillofacial surgeries.

Original languageEnglish
Pages (from-to)387-396
Number of pages10
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume81
Issue number2
DOIs
Publication statusPublished - 1 May 2007
Externally publishedYes

Fingerprint

Nanocomposites
Osteoblasts
Calcium phosphate
Gene expression
Silica
Gene Expression
Bone
Bearings (structural)
Compressive Strength
Oral Surgery
Porosity
Weight-Bearing
Orthopedics
Surgery
Compressive strength
Tissue Scaffolds
Bone and Bones
Mechanical properties
Tissue culture
X Ray Computed Tomography

Keywords

  • Mechanical properties
  • Osteoblast gene expression
  • Porous nanocomposites
  • Tissue engineering
  • X-ray tomography

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Enhancement of osteoblast gene expression by mechanically compatible porous Si-rich nanocomposite. / Gupta, Gautam; El-Ghannam, Ahmed; Kirakodu, Sreenatha; Khraisheh, Marwan; Zbib, Hussein.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 81, No. 2, 01.05.2007, p. 387-396.

Research output: Contribution to journalArticle

@article{0cf2d9c17e544503a36d74fbc63d450a,
title = "Enhancement of osteoblast gene expression by mechanically compatible porous Si-rich nanocomposite",
abstract = "Synthesis of a porous bioactive ceramic implant for load bearing applications is a challenging task in maxillofacial and orthopedic surgeries. A novel bioactive resorbable silica-calcium phosphate nanocomposite (SCPC) has recently been introduced as a potential bone graft. In the present study, we employed SCPC to develop a resorbable porous scaffold and analyzed the effects of composition and porosity on the mechanical properties. The ranges of compressive strength and modulus of elasticity of SCPC containing 32-56{\%} porosity were 1.5-50 MPa and 0.14-2.1 GPa, respectively, which matched the corresponding values for trabecular bone. The compressive strength of dense SCPC was dependent on the Si content and acquired values (93-285 MPa) comparable to that of cortical bone. The superior mechanical properties of SCPC are attributed to the intricate interactions at the boundaries of the nanograins and to the homogenous distribution of hierarchical pore-structure throughout the material volume. X-ray computed tomography and mercury porosimetry analyses revealed high interconnectivity of the pores in the size range 3 nm to 650 μm. Quantitative real-time PCR analyses showed that neonatal rat calvarial osteoblasts attached to Si-rich SCPC expressed 5- and 26-fold higher osteocalcin mRNA levels compared to cells attached to ProOsteon™ hydroxyapatite disks and tissue culture polystyrene plates respectively, after four days in culture. Results of the present study strongly suggest that porous, bioactive resorbable SCPCs can serve as tissue engineering scaffolds for cell delivery to treat load-bearing bone defects in orthopedic and maxillofacial surgeries.",
keywords = "Mechanical properties, Osteoblast gene expression, Porous nanocomposites, Tissue engineering, X-ray tomography",
author = "Gautam Gupta and Ahmed El-Ghannam and Sreenatha Kirakodu and Marwan Khraisheh and Hussein Zbib",
year = "2007",
month = "5",
day = "1",
doi = "10.1002/jbm.b.30675",
language = "English",
volume = "81",
pages = "387--396",
journal = "Journal of Biomedical Materials Research - Part B Applied Biomaterials",
issn = "1552-4973",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

TY - JOUR

T1 - Enhancement of osteoblast gene expression by mechanically compatible porous Si-rich nanocomposite

AU - Gupta, Gautam

AU - El-Ghannam, Ahmed

AU - Kirakodu, Sreenatha

AU - Khraisheh, Marwan

AU - Zbib, Hussein

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Synthesis of a porous bioactive ceramic implant for load bearing applications is a challenging task in maxillofacial and orthopedic surgeries. A novel bioactive resorbable silica-calcium phosphate nanocomposite (SCPC) has recently been introduced as a potential bone graft. In the present study, we employed SCPC to develop a resorbable porous scaffold and analyzed the effects of composition and porosity on the mechanical properties. The ranges of compressive strength and modulus of elasticity of SCPC containing 32-56% porosity were 1.5-50 MPa and 0.14-2.1 GPa, respectively, which matched the corresponding values for trabecular bone. The compressive strength of dense SCPC was dependent on the Si content and acquired values (93-285 MPa) comparable to that of cortical bone. The superior mechanical properties of SCPC are attributed to the intricate interactions at the boundaries of the nanograins and to the homogenous distribution of hierarchical pore-structure throughout the material volume. X-ray computed tomography and mercury porosimetry analyses revealed high interconnectivity of the pores in the size range 3 nm to 650 μm. Quantitative real-time PCR analyses showed that neonatal rat calvarial osteoblasts attached to Si-rich SCPC expressed 5- and 26-fold higher osteocalcin mRNA levels compared to cells attached to ProOsteon™ hydroxyapatite disks and tissue culture polystyrene plates respectively, after four days in culture. Results of the present study strongly suggest that porous, bioactive resorbable SCPCs can serve as tissue engineering scaffolds for cell delivery to treat load-bearing bone defects in orthopedic and maxillofacial surgeries.

AB - Synthesis of a porous bioactive ceramic implant for load bearing applications is a challenging task in maxillofacial and orthopedic surgeries. A novel bioactive resorbable silica-calcium phosphate nanocomposite (SCPC) has recently been introduced as a potential bone graft. In the present study, we employed SCPC to develop a resorbable porous scaffold and analyzed the effects of composition and porosity on the mechanical properties. The ranges of compressive strength and modulus of elasticity of SCPC containing 32-56% porosity were 1.5-50 MPa and 0.14-2.1 GPa, respectively, which matched the corresponding values for trabecular bone. The compressive strength of dense SCPC was dependent on the Si content and acquired values (93-285 MPa) comparable to that of cortical bone. The superior mechanical properties of SCPC are attributed to the intricate interactions at the boundaries of the nanograins and to the homogenous distribution of hierarchical pore-structure throughout the material volume. X-ray computed tomography and mercury porosimetry analyses revealed high interconnectivity of the pores in the size range 3 nm to 650 μm. Quantitative real-time PCR analyses showed that neonatal rat calvarial osteoblasts attached to Si-rich SCPC expressed 5- and 26-fold higher osteocalcin mRNA levels compared to cells attached to ProOsteon™ hydroxyapatite disks and tissue culture polystyrene plates respectively, after four days in culture. Results of the present study strongly suggest that porous, bioactive resorbable SCPCs can serve as tissue engineering scaffolds for cell delivery to treat load-bearing bone defects in orthopedic and maxillofacial surgeries.

KW - Mechanical properties

KW - Osteoblast gene expression

KW - Porous nanocomposites

KW - Tissue engineering

KW - X-ray tomography

UR - http://www.scopus.com/inward/record.url?scp=34247565961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247565961&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.30675

DO - 10.1002/jbm.b.30675

M3 - Article

VL - 81

SP - 387

EP - 396

JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials

JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials

SN - 1552-4973

IS - 2

ER -