Enhanced monocyte response and decreased central memory T cells in children with invasive Staphylococcus aureus infections

Monica I. Ardura, Romain Banchereau, Asuncion Mejias, Tiziana Di Pucchio, Casey Glaser, Florence Allantaz, Virginia Pascual, Jacques Banchereau, Damien J. Chaussabel, Octavio Ramilo

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Staphylococcus aureus has emerged as a significant pathogen causing severe invasive disease in otherwise healthy people. Despite considerable advances in understanding the epidemiology, resistance mechanisms, and virulence factors produced by the bacteria, there is limited knowledge of the in vivo host immune response to acute, invasive S. aureus infections. Herein, we report that peripheral blood mononuclear cells from patients with severe S. aureus infections demonstrate a distinctive and robust gene expression profile which is validated in a distinct group of patients and on a different microarray platform. Application of a systems-wide modular analysis framework reveals significant over-expression of innate immunity genes and under-expression of genes related to adaptive immunity. Simultaneous flow cytometry analyses demonstrated marked alterations in immune cell numbers, with decreased central memory CD4 and CD8 T cells and increased numbers of monocytes. CD14+ monocyte numbers significantly correlated with the gene expression levels of genes related to the innate immune response. These results demonstrate the value of applying a systems biology approach that reveals the significant alterations in the components of circulating blood lymphocytes and monocytes in invasive S. aureus infections.

Original languageEnglish
Article numbere5446
JournalPLoS One
Volume4
Issue number5
DOIs
Publication statusPublished - 8 May 2009
Externally publishedYes

Fingerprint

T-cells
monocytes
Staphylococcus aureus
Monocytes
T-lymphocytes
Genes
T-Lymphocytes
Data storage equipment
Gene expression
Blood
Infection
Innate Immunity
infection
gene expression
Epidemiology
Lymphocytes
Flow cytometry
Cell Count
R Factors
Virulence Factors

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Ardura, M. I., Banchereau, R., Mejias, A., Di Pucchio, T., Glaser, C., Allantaz, F., ... Ramilo, O. (2009). Enhanced monocyte response and decreased central memory T cells in children with invasive Staphylococcus aureus infections. PLoS One, 4(5), [e5446]. https://doi.org/10.1371/journal.pone.0005446

Enhanced monocyte response and decreased central memory T cells in children with invasive Staphylococcus aureus infections. / Ardura, Monica I.; Banchereau, Romain; Mejias, Asuncion; Di Pucchio, Tiziana; Glaser, Casey; Allantaz, Florence; Pascual, Virginia; Banchereau, Jacques; Chaussabel, Damien J.; Ramilo, Octavio.

In: PLoS One, Vol. 4, No. 5, e5446, 08.05.2009.

Research output: Contribution to journalArticle

Ardura, MI, Banchereau, R, Mejias, A, Di Pucchio, T, Glaser, C, Allantaz, F, Pascual, V, Banchereau, J, Chaussabel, DJ & Ramilo, O 2009, 'Enhanced monocyte response and decreased central memory T cells in children with invasive Staphylococcus aureus infections', PLoS One, vol. 4, no. 5, e5446. https://doi.org/10.1371/journal.pone.0005446
Ardura, Monica I. ; Banchereau, Romain ; Mejias, Asuncion ; Di Pucchio, Tiziana ; Glaser, Casey ; Allantaz, Florence ; Pascual, Virginia ; Banchereau, Jacques ; Chaussabel, Damien J. ; Ramilo, Octavio. / Enhanced monocyte response and decreased central memory T cells in children with invasive Staphylococcus aureus infections. In: PLoS One. 2009 ; Vol. 4, No. 5.
@article{ed3701631e634da5987a707e9f7f9a87,
title = "Enhanced monocyte response and decreased central memory T cells in children with invasive Staphylococcus aureus infections",
abstract = "Staphylococcus aureus has emerged as a significant pathogen causing severe invasive disease in otherwise healthy people. Despite considerable advances in understanding the epidemiology, resistance mechanisms, and virulence factors produced by the bacteria, there is limited knowledge of the in vivo host immune response to acute, invasive S. aureus infections. Herein, we report that peripheral blood mononuclear cells from patients with severe S. aureus infections demonstrate a distinctive and robust gene expression profile which is validated in a distinct group of patients and on a different microarray platform. Application of a systems-wide modular analysis framework reveals significant over-expression of innate immunity genes and under-expression of genes related to adaptive immunity. Simultaneous flow cytometry analyses demonstrated marked alterations in immune cell numbers, with decreased central memory CD4 and CD8 T cells and increased numbers of monocytes. CD14+ monocyte numbers significantly correlated with the gene expression levels of genes related to the innate immune response. These results demonstrate the value of applying a systems biology approach that reveals the significant alterations in the components of circulating blood lymphocytes and monocytes in invasive S. aureus infections.",
author = "Ardura, {Monica I.} and Romain Banchereau and Asuncion Mejias and {Di Pucchio}, Tiziana and Casey Glaser and Florence Allantaz and Virginia Pascual and Jacques Banchereau and Chaussabel, {Damien J.} and Octavio Ramilo",
year = "2009",
month = "5",
day = "8",
doi = "10.1371/journal.pone.0005446",
language = "English",
volume = "4",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

TY - JOUR

T1 - Enhanced monocyte response and decreased central memory T cells in children with invasive Staphylococcus aureus infections

AU - Ardura, Monica I.

AU - Banchereau, Romain

AU - Mejias, Asuncion

AU - Di Pucchio, Tiziana

AU - Glaser, Casey

AU - Allantaz, Florence

AU - Pascual, Virginia

AU - Banchereau, Jacques

AU - Chaussabel, Damien J.

AU - Ramilo, Octavio

PY - 2009/5/8

Y1 - 2009/5/8

N2 - Staphylococcus aureus has emerged as a significant pathogen causing severe invasive disease in otherwise healthy people. Despite considerable advances in understanding the epidemiology, resistance mechanisms, and virulence factors produced by the bacteria, there is limited knowledge of the in vivo host immune response to acute, invasive S. aureus infections. Herein, we report that peripheral blood mononuclear cells from patients with severe S. aureus infections demonstrate a distinctive and robust gene expression profile which is validated in a distinct group of patients and on a different microarray platform. Application of a systems-wide modular analysis framework reveals significant over-expression of innate immunity genes and under-expression of genes related to adaptive immunity. Simultaneous flow cytometry analyses demonstrated marked alterations in immune cell numbers, with decreased central memory CD4 and CD8 T cells and increased numbers of monocytes. CD14+ monocyte numbers significantly correlated with the gene expression levels of genes related to the innate immune response. These results demonstrate the value of applying a systems biology approach that reveals the significant alterations in the components of circulating blood lymphocytes and monocytes in invasive S. aureus infections.

AB - Staphylococcus aureus has emerged as a significant pathogen causing severe invasive disease in otherwise healthy people. Despite considerable advances in understanding the epidemiology, resistance mechanisms, and virulence factors produced by the bacteria, there is limited knowledge of the in vivo host immune response to acute, invasive S. aureus infections. Herein, we report that peripheral blood mononuclear cells from patients with severe S. aureus infections demonstrate a distinctive and robust gene expression profile which is validated in a distinct group of patients and on a different microarray platform. Application of a systems-wide modular analysis framework reveals significant over-expression of innate immunity genes and under-expression of genes related to adaptive immunity. Simultaneous flow cytometry analyses demonstrated marked alterations in immune cell numbers, with decreased central memory CD4 and CD8 T cells and increased numbers of monocytes. CD14+ monocyte numbers significantly correlated with the gene expression levels of genes related to the innate immune response. These results demonstrate the value of applying a systems biology approach that reveals the significant alterations in the components of circulating blood lymphocytes and monocytes in invasive S. aureus infections.

UR - http://www.scopus.com/inward/record.url?scp=65549116870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65549116870&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0005446

DO - 10.1371/journal.pone.0005446

M3 - Article

C2 - 19424507

AN - SCOPUS:65549116870

VL - 4

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 5

M1 - e5446

ER -