Enhanced humoral and mucosal immune responses after intranasal immunization with chimeric multiple antigen peptide of LcrV antigen epitopes of Yersinia pestis coupled to palmitate in mice

Sri Jayaprakash Babu Uppada, Ajaz Ahmad Bhat, Anil Sah, Rao Nageswara Donthamshetty

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Yersinia pestis is the causative agent of the most deadly disease plague. F1 and V antigens are the major vaccine candidates. Six protective epitopes of V antigen of varying length (15-25aa) were assembled on a lysine backbone as multiple antigen peptide (MAP) using standard Fmoc chemistry. Palmitate was coupled at amino terminus end. Amino acid analysis, SDS-PAGE, immunoblot and immunoreactivity proved the authenticity of MAP. MAP was immunized intranasally encapsulated in PLGA (polylactide-co-glycolide) microspheres and with/without/adjuvants murabutide and CpG ODN 1826 (CpG), in three strains of mice. Humoral and mucosal immune responses were studied till day 120 and memory response was checked after immunization with native V antigen on day 120. Epitope specific serum and mucosal washes IgG, IgA, IgG subclasses and specific activity were measured by indirect ELISA and sandwich ELISA, respectively. IgG and IgA peak antibody titers of all the MAP construct formulations in sera were ranging from 71,944 to 360,578 and 4493 to 28,644, respectively. MAP with CpG showed significantly high (. p<. 0.0001) antibody titers ranging from 101,690 to 360,578 for IgG and 28,644 for IgA. Mucosal peak IgG and IgA titers were ranging from 1425 to 8072 and 1425 to 7183, respectively in intestinal washes and 799-4528 and 566-4027, respectively in lung washes. MAP with CpG showed significantly high (. p<. 0.001) SIgA titers of 8000 in lung and 16,000 in intestinal washes. IgG isotyping revealed IgG2a/IgG1 ratio. >. 1 with CpG. Serum and mucosal antipeptide IgG and IgA specific activities correlated well with antibody titers. All the constituent peptides contributed towards immune response. Structural analysis of MAP revealed little or no interaction between the peptides. Present study showed MAP to be highly immunogenic with high and long lasting antibody titers in serum and mucosal washes with good recall response with/without CpG as an adjuvant which can be used for vaccine development for plague.

Original languageEnglish
Pages (from-to)9352-9360
Number of pages9
JournalVaccine
Volume29
Issue number50
DOIs
Publication statusPublished - 21 Nov 2011
Externally publishedYes

Fingerprint

Yersinia pestis
mucosal immunity
Mucosal Immunity
Palmitates
palmitates
Humoral Immunity
humoral immunity
epitopes
Epitopes
Immunization
immunization
peptides
antigens
Antigens
Peptides
mice
Immunoglobulin G
Immunoglobulin A
plague
Serum

Keywords

  • Adjuvant
  • CpG ODN
  • MAP construct
  • Mucosal immunity
  • V antigen
  • Yersinia

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Enhanced humoral and mucosal immune responses after intranasal immunization with chimeric multiple antigen peptide of LcrV antigen epitopes of Yersinia pestis coupled to palmitate in mice. / Uppada, Sri Jayaprakash Babu; Bhat, Ajaz Ahmad; Sah, Anil; Donthamshetty, Rao Nageswara.

In: Vaccine, Vol. 29, No. 50, 21.11.2011, p. 9352-9360.

Research output: Contribution to journalArticle

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AU - Sah, Anil

AU - Donthamshetty, Rao Nageswara

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