Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells

Bi Sen Ding, Kazunori Gomi, Shahin Rafii, Ronald Crystal, Matthew S. Walters

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Human airway basal cells are the stem(or progenitor) population of the airway epithelium, and play a central role in anchoring the epithelium to the basement membrane. The anatomic position of basal cells allows for potential paracrine signaling between themand the underlying non-epithelial stromal cells. In support of this, we have previously demonstrated that endothelial cells support growth of basal cells during co-culture through vascular endothelial growth factor A (VEGFA)-mediated signaling. Building on these findings, we found, by RNA sequencing analysis, that basal cells expressed multiple fibroblast growth factor (FGF) ligands (FGF2, FGF5, FGF11 and FGF13) and that only FGF2 and FGF5 were capable of functioning in a paracrine manner to activate classical FGF receptor (FGFR) signaling. Antibody-mediated blocking of FGFR1 during basal-cell-endothelial-cell co-culture significantly reduced the endothelial-cell-dependent basal cellgrowth. Stimulationof endothelial cellswith basal-cell-derived growth factors induced endothelial cell expression of matrix metallopeptidase 14 (MMP14), and short hairpin RNA (shRNA)- mediated knockdown of endothelial cell MMP14 significantly reduced the endothelial-cell-dependentgrowth ofbasal cells.Overall, these data characterize a new growth-factor-mediated reciprocal 'crosstalk' between human airway basal cells and endothelial cells that regulates proliferation of basal cells.

Original languageEnglish
Pages (from-to)2983-2988
Number of pages6
JournalJournal of Cell Science
Volume128
Issue number15
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Fingerprint

Metalloproteases
Endothelial Cells
Growth
Fibroblast Growth Factor 2
Coculture Techniques
Intercellular Signaling Peptides and Proteins
Stem Cells
Epithelium
Paracrine Communication
RNA Sequence Analysis
Fibroblast Growth Factor Receptors
Blocking Antibodies
Fibroblast Growth Factors
Stromal Cells
Basement Membrane
Vascular Endothelial Growth Factor A
Small Interfering RNA
Cell Culture Techniques
Cell Proliferation
Ligands

Keywords

  • Airway basal cell
  • Crosstalk
  • Endothelial cell
  • MMP14
  • Progenitor cell

ASJC Scopus subject areas

  • Cell Biology

Cite this

Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. / Ding, Bi Sen; Gomi, Kazunori; Rafii, Shahin; Crystal, Ronald; Walters, Matthew S.

In: Journal of Cell Science, Vol. 128, No. 15, 01.01.2015, p. 2983-2988.

Research output: Contribution to journalArticle

Ding, Bi Sen ; Gomi, Kazunori ; Rafii, Shahin ; Crystal, Ronald ; Walters, Matthew S. / Endothelial MMP14 is required for endothelial-dependent growth support of human airway basal cells. In: Journal of Cell Science. 2015 ; Vol. 128, No. 15. pp. 2983-2988.
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