Endoplasmic reticulum form of calreticulin modulates glucocorticoid- sensitive gene expression

Marek Michalak, Kimberly Burns, Christi Andrin, Nasrin Mesaeli, Greta H. Jass, Jody L. Busaan, Michal Opas

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Calreticulin is a ubiquitously expressed Ca2+-binding protein of the endoplasmic reticulum (ER), which inhibits DNA binding in vitro and transcriptional activation in vivo by steroid hormone receptors. Transient transfection assays were carried out to investigate the effects of different intracellular targeting of calreticulin on transactivation mediated by glucocorticoid receptor. BSC40 cells were transfected with either calreticulin expression vector (ER form of calreticulin) or calreticulin expression vector encoding calreticulin minus leader peptide, resulting in cytoplasmic localization of the recombinant protein. Transfection of BSC40 cells with calreticulin expression vector encoding the ER form of the protein led to 40-50% inhibition of the dexamethasone-sensitive stimulation of luciferase expression. However, in a similar experiment, but using the calreticulin expression vector encoding cytoplasmic calreticulin, dexamethasone-stimulated activation of the luciferase reporter gene was inhibited by only 10%. We conclude that the ER, but not cytosolic, form of calreticulin is responsible for inhibition of glucocorticoid receptor- mediated gene expression. These effects are specific to calreticulin, since overexpression of the ER lumenal proteins (BiP, ERp72, or calsequestrin) has no effect on glucocorticoid-sensitive gene expression. The N domain of calreticulin binds to the DNA binding domain of the glucocorticoid receptor in vitro; however, we show that the N+P domain of calreticulin, when synthesized without the ER signal sequence, does not inhibit glucocorticoid receptor function in vivo. Furthermore, expression of the N domain of calreticulin and the DNA binding domain of glucocorticoid receptor as fusion proteins with GAL4 in the yeast two-hybrid system revealed that calreticulin does not interact with glucocorticoid receptor under these conditions. We conclude that calreticulin and glucocorticoid receptor may not interact in vivo and that the calreticulin-dependent modulation of the glucocorticoid receptor function may therefore be due to a calreticulin-dependent signaling from the ER.

Original languageEnglish
Pages (from-to)29436-29445
Number of pages10
JournalJournal of Biological Chemistry
Volume271
Issue number46
DOIs
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Calreticulin
Gene expression
Endoplasmic Reticulum
Glucocorticoids
Gene Expression
Glucocorticoid Receptors
Protein Sorting Signals
Luciferases
Dexamethasone
Transcriptional Activation
Transfection
DNA
Chemical activation
Steroid hormones
Calsequestrin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Endoplasmic reticulum form of calreticulin modulates glucocorticoid- sensitive gene expression. / Michalak, Marek; Burns, Kimberly; Andrin, Christi; Mesaeli, Nasrin; Jass, Greta H.; Busaan, Jody L.; Opas, Michal.

In: Journal of Biological Chemistry, Vol. 271, No. 46, 1996, p. 29436-29445.

Research output: Contribution to journalArticle

Michalak, Marek ; Burns, Kimberly ; Andrin, Christi ; Mesaeli, Nasrin ; Jass, Greta H. ; Busaan, Jody L. ; Opas, Michal. / Endoplasmic reticulum form of calreticulin modulates glucocorticoid- sensitive gene expression. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 46. pp. 29436-29445.
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abstract = "Calreticulin is a ubiquitously expressed Ca2+-binding protein of the endoplasmic reticulum (ER), which inhibits DNA binding in vitro and transcriptional activation in vivo by steroid hormone receptors. Transient transfection assays were carried out to investigate the effects of different intracellular targeting of calreticulin on transactivation mediated by glucocorticoid receptor. BSC40 cells were transfected with either calreticulin expression vector (ER form of calreticulin) or calreticulin expression vector encoding calreticulin minus leader peptide, resulting in cytoplasmic localization of the recombinant protein. Transfection of BSC40 cells with calreticulin expression vector encoding the ER form of the protein led to 40-50{\%} inhibition of the dexamethasone-sensitive stimulation of luciferase expression. However, in a similar experiment, but using the calreticulin expression vector encoding cytoplasmic calreticulin, dexamethasone-stimulated activation of the luciferase reporter gene was inhibited by only 10{\%}. We conclude that the ER, but not cytosolic, form of calreticulin is responsible for inhibition of glucocorticoid receptor- mediated gene expression. These effects are specific to calreticulin, since overexpression of the ER lumenal proteins (BiP, ERp72, or calsequestrin) has no effect on glucocorticoid-sensitive gene expression. The N domain of calreticulin binds to the DNA binding domain of the glucocorticoid receptor in vitro; however, we show that the N+P domain of calreticulin, when synthesized without the ER signal sequence, does not inhibit glucocorticoid receptor function in vivo. Furthermore, expression of the N domain of calreticulin and the DNA binding domain of glucocorticoid receptor as fusion proteins with GAL4 in the yeast two-hybrid system revealed that calreticulin does not interact with glucocorticoid receptor under these conditions. We conclude that calreticulin and glucocorticoid receptor may not interact in vivo and that the calreticulin-dependent modulation of the glucocorticoid receptor function may therefore be due to a calreticulin-dependent signaling from the ER.",
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