Elucidating the stimulatory and inhibitory effects of dissolved sulfide on sulfur-oxidizing bacteria (SOB) driven autotrophic denitrification

Hui Lu, Haiqin Huang, Weiming Yang, Hamish Mackey, Samir Kumar Khanal, Di Wu, Guang Hao Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Autotrophic denitrification has been widely studied for odor mitigation, corrosion control and nitrogen removal in recent years. This paper examines the response of sulfur-oxidizing bacteria (SOB) driven autotrophic denitrification under short-term stress of dissolved sulfide. A series of batch tests were conducted to investigate the effect of different sulfide concentrations (0–1600 mg-total dissolved sulfide (TDS)/L) on autotrophic denitrification and sulfide oxidation by SOB-enriched sludge. Our results show that autotrophic denitrification (NO3 to N2) was stimulated up to 200 mg-TDS/L with a maximum denitrification rate of 9.4 mg-N/g-volatile suspended solids (VSS)/h, and the nitrite reduction was a rate limiting step. When sulfide concentration was higher than 200 mg-TDS/L, it inhibited nitrate reductase, and nitrate reduction became the rate limiting step according to Edwards and Aiba inhibition models. Sulfide oxidation, however, was not inhibited and the maximum rate of 100.3 mg-TDS/g-VSS/h was obtained at sulfide concentration of 1000 mg-TDS/L. It is important to point out that the transient inhibition on autotrophic denitrification caused by high sulfide stress was resilient and non-lethal with no significant changes in cell viability even under sulfide concentration of 1000 mg-TDS/L. This study reveals the stimulatory and inhibitory effects of dissolved sulfide on SOB driven autotrophic denitrification and its possible underlying mechanism with discussion on engineering implications.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalWater Research
Volume133
DOIs
Publication statusPublished - 15 Apr 2018

Fingerprint

Denitrification
denitrification
Bacteria
Sulfur
sulfur
sulfide
bacterium
effect
Sulfides
Nitrates
nitrate
corrosion control
oxidation
Oxidation
Nitrogen removal
Odors
odor
nitrite

Keywords

  • Autotrophic denitrification
  • Biological sulfide oxidation
  • Dissolved sulfide
  • Sulfur-oxidizing bacteria (SOB)

ASJC Scopus subject areas

  • Ecological Modelling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

Elucidating the stimulatory and inhibitory effects of dissolved sulfide on sulfur-oxidizing bacteria (SOB) driven autotrophic denitrification. / Lu, Hui; Huang, Haiqin; Yang, Weiming; Mackey, Hamish; Khanal, Samir Kumar; Wu, Di; Chen, Guang Hao.

In: Water Research, Vol. 133, 15.04.2018, p. 165-172.

Research output: Contribution to journalArticle

Lu, Hui ; Huang, Haiqin ; Yang, Weiming ; Mackey, Hamish ; Khanal, Samir Kumar ; Wu, Di ; Chen, Guang Hao. / Elucidating the stimulatory and inhibitory effects of dissolved sulfide on sulfur-oxidizing bacteria (SOB) driven autotrophic denitrification. In: Water Research. 2018 ; Vol. 133. pp. 165-172.
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