Elevated expression of CYP1A1 and γ-SYNUCLEIN in human ectopic (ovarian) endometriosis compared with eutopic endometrium

Maneesh N. Singh, Helen F. Stringfellow, Siân E. Taylor, Kate M. Ashton, Mushfika Ahmad, Khalil R. Abdo, Omar Ali El-Agnaf, Pierre L. Martin-Hirsch, Francis L. Martin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Endometriosis is a debilitating disease in which apoptotic, genetic, immunological, angiogenic and environmental factors have been implicated. Endocrine-disrupting agents (e.g. dioxins) might be involved. Dioxins, via the arylhydrocarbon receptor (AhR), induce estrogen-metabolizing enzymes CYP1A1 and CYP1B1. Elevated expression of γ-SYNUCLEIN (γ-SYN) has been associated with hormone-related conditions. Tissue sets consisting of eutopic and ectopic (ovarian) endometrium from patients with stage 3 or 4 endometriosis were obtained. Following RNA extraction and reverse transcription, quantitative real-time reverse transcriptase-polymerase chain reaction was performed for anti-apoptotic B-cell leukaemia/lymphoma 2 (BCL-2), CYP1A1, CYP1B1, estrogen receptor (ER)α, ERβ and γ-SYN. Immunohistochemical analyses for γ-syn, ERα, ERβ and CYP1A1 were also conducted. A 3-9-fold increase in intra-individual expression of CYP1A1 in ectopic (ovarian) endometrium compared with eutopic tissue was observed; immunohistochemical analyses pointed to CYP1A1 being localized to the glandular epithelium. This intra-individual expression profile was not observed for CYP1B1 or BCL-2. However, a 5-53-fold intra-individual increase in γ-SYN expression was also demonstrated in six of nine tissue sets (a further two showed an increase that was not considered significant) when comparing ectopic to eutopic endometrium; γ-syn positivity was associated with endothelial cells. An elevation in ERβ was also noted when comparing ectopic to eutopic endometrium; with regard to ERα, this was inconsistent. These results suggest an up-regulation of dioxin-inducible CYP1A1 and γ-SYN occurs in endometriosis. Whether γ-syn may be a novel diagnostic marker for endometriosis remains to be ascertained.

Original languageEnglish
Pages (from-to)655-663
Number of pages9
JournalMolecular Human Reproduction
Volume14
Issue number11
DOIs
Publication statusPublished - 2008
Externally publishedYes

Fingerprint

Cytochrome P-450 CYP1A1
Endometriosis
Endometrium
Estrogen Receptors
Dioxins
B-Cell Leukemia
B-Cell Lymphoma
Angiogenesis Inducing Agents
Reverse Transcriptase Polymerase Chain Reaction
Reverse Transcription
Real-Time Polymerase Chain Reaction
Up-Regulation
Epithelium
Endothelial Cells
Hormones
RNA
Enzymes

Keywords

  • γ-synuclein
  • BCL-2
  • CYP1A1
  • Endometriosis
  • Estrogen receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Embryology
  • Cell Biology
  • Genetics
  • Developmental Biology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Singh, M. N., Stringfellow, H. F., Taylor, S. E., Ashton, K. M., Ahmad, M., Abdo, K. R., ... Martin, F. L. (2008). Elevated expression of CYP1A1 and γ-SYNUCLEIN in human ectopic (ovarian) endometriosis compared with eutopic endometrium. Molecular Human Reproduction, 14(11), 655-663. https://doi.org/10.1093/molehr/gan056

Elevated expression of CYP1A1 and γ-SYNUCLEIN in human ectopic (ovarian) endometriosis compared with eutopic endometrium. / Singh, Maneesh N.; Stringfellow, Helen F.; Taylor, Siân E.; Ashton, Kate M.; Ahmad, Mushfika; Abdo, Khalil R.; Ali El-Agnaf, Omar; Martin-Hirsch, Pierre L.; Martin, Francis L.

In: Molecular Human Reproduction, Vol. 14, No. 11, 2008, p. 655-663.

Research output: Contribution to journalArticle

Singh, MN, Stringfellow, HF, Taylor, SE, Ashton, KM, Ahmad, M, Abdo, KR, Ali El-Agnaf, O, Martin-Hirsch, PL & Martin, FL 2008, 'Elevated expression of CYP1A1 and γ-SYNUCLEIN in human ectopic (ovarian) endometriosis compared with eutopic endometrium', Molecular Human Reproduction, vol. 14, no. 11, pp. 655-663. https://doi.org/10.1093/molehr/gan056
Singh, Maneesh N. ; Stringfellow, Helen F. ; Taylor, Siân E. ; Ashton, Kate M. ; Ahmad, Mushfika ; Abdo, Khalil R. ; Ali El-Agnaf, Omar ; Martin-Hirsch, Pierre L. ; Martin, Francis L. / Elevated expression of CYP1A1 and γ-SYNUCLEIN in human ectopic (ovarian) endometriosis compared with eutopic endometrium. In: Molecular Human Reproduction. 2008 ; Vol. 14, No. 11. pp. 655-663.
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