Efficient presentation of multivalent antigens targeted to various cell surface molecules of dendritic cells and surface Ig of antigen-specific B cells

Karine Serre, Patrick Machy, Jean-Charles B. Grivel, Gilles Jolly, Nicole Brun, Jacques Barbet, Lee Leserman

Research output: Contribution to journalArticle

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Abstract

To study the relation between the form of an Ag and the response to it, we compared presentation in vitro with hen egg lysozyme (HEL)-specific T cells from TCR transgenic mice of free HEL and liposome-encapsulated HEL by different APC. HEL-specific splenic B cells or bone marrow-derived dendritic cells were incubated with free HEL or HEL-containing liposomes targeted by Ab to either surface Ig, the Fc receptor, or MHC class I and II molecules, Ag presentation by HEL-specific B cells was at least 100-fold more efficient for HEL in surface Ig-targeted liposomes than free HEL taken up by the same receptor or HEL in liposomes targeted to class I or II molecules. Ag presentation by dendritic cells from Fc receptor-targeted vesicles was augmented 1,000-10,000-fold compared with free Ag or nontargeted liposomes, but presentation was also efficient when Ag was targeted to class I or II molecules. These results indicate that Ag-specific B cells and dendritic cells can be equally efficient in stimulating IL-2 production by Ag-specific T cells from unimmunized TCR transgenic mice when the Ag is multivalent and taken up by appropriate receptors. In contrast to B cells, which require engagement of surface Ig for optimal presentation, dendritic cells may present Ag by means of several different cell surface molecules.

Original languageEnglish
Pages (from-to)6059-6067
Number of pages9
JournalJournal of Immunology
Volume161
Issue number11
Publication statusPublished - 1 Dec 1998
Externally publishedYes

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Antigen Presentation
Surface Antigens
Dendritic Cells
B-Lymphocytes
Liposomes
Fc Receptors
Transgenic Mice
hen egg lysozyme
T-Lymphocytes
Interleukin-2
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

Efficient presentation of multivalent antigens targeted to various cell surface molecules of dendritic cells and surface Ig of antigen-specific B cells. / Serre, Karine; Machy, Patrick; Grivel, Jean-Charles B.; Jolly, Gilles; Brun, Nicole; Barbet, Jacques; Leserman, Lee.

In: Journal of Immunology, Vol. 161, No. 11, 01.12.1998, p. 6059-6067.

Research output: Contribution to journalArticle

Serre, Karine ; Machy, Patrick ; Grivel, Jean-Charles B. ; Jolly, Gilles ; Brun, Nicole ; Barbet, Jacques ; Leserman, Lee. / Efficient presentation of multivalent antigens targeted to various cell surface molecules of dendritic cells and surface Ig of antigen-specific B cells. In: Journal of Immunology. 1998 ; Vol. 161, No. 11. pp. 6059-6067.
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