Efficient Monte Carlo simulation of confocal microscopy in biological tissue

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Abstract

A variance-reduction technique is described that greatly improves the efficiency of Monte Carlo simulations of reflection-mode confocal microscopy in anisotropically scattering media. The efficiency gain is large enough that the performance of confocal microscopes probing as deep as 5 scattering lengths can be simulated with a desktop computer. We use the technique to simulate the response of a true confocal microscope probing biological tissue, a problem that has been impractical to undertake by using conventional Monte Carlo methods. Our most important finding is that operation of a confocal microscope in the true confocal mode enables much more effective rejection of undesired scattered light than operation in the partially coherent mode, but the maximum probing depths of microscopes operated in either mode are similar (2–3 scattering lengths) in practice because of sensitivity limitations.

Original languageEnglish
Pages (from-to)952-961
Number of pages10
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Volume13
Issue number5
DOIs
Publication statusPublished - 1996
Externally publishedYes

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Confocal microscopy
Microscopes
microscopes
Tissue
microscopy
Scattering
simulation
scattering
Laser modes
Personal computers
rejection
Monte Carlo method
Monte Carlo methods
Monte Carlo simulation
sensitivity

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Computer Vision and Pattern Recognition

Cite this

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AB - A variance-reduction technique is described that greatly improves the efficiency of Monte Carlo simulations of reflection-mode confocal microscopy in anisotropically scattering media. The efficiency gain is large enough that the performance of confocal microscopes probing as deep as 5 scattering lengths can be simulated with a desktop computer. We use the technique to simulate the response of a true confocal microscope probing biological tissue, a problem that has been impractical to undertake by using conventional Monte Carlo methods. Our most important finding is that operation of a confocal microscope in the true confocal mode enables much more effective rejection of undesired scattered light than operation in the partially coherent mode, but the maximum probing depths of microscopes operated in either mode are similar (2–3 scattering lengths) in practice because of sensitivity limitations.

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