Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5

Ester Ballana, Jing Wang, Frédéric Venail, Xavier P. Estivill, Jean Luc Puel, Maria L. Arbonès, Assumpció Bosch

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Congenital deafness, affecting 1 in 1000 neonates, can lead to major problems in speech, cognitive and psychosocial development. Congenital deafness is mainly caused by mutations in connexins, hemi-channel proteins forming gap-junctions between supporting cells in the sensory epithelia. We describe a high tropism of AAV5 serotype for the supporting cells of the cochlea, both in vitro in postnatal day 4 mouse explants, and in vivo in the adult guinea-pig inner ear, through scala media perfusion. AAV5 transduction correlates with PDGFRα expression, previously reported as AAV5 receptor. This vector could be of major interest in addressing gene therapy approaches to deafness as well as for studying basic aspects of inner-ear development and hearing mechanisms.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalNeuroscience Letters
Volume442
Issue number2
DOIs
Publication statusPublished - 12 Sep 2008
Externally publishedYes

Fingerprint

Dependovirus
Cochlea
Deafness
Connexins
Inner Ear
Cochlear Duct
Tropism
Genetic Therapy
Hearing
Guinea Pigs
Epithelium
Perfusion
Mutation
Serogroup

Keywords

  • AAV5 serotype
  • Gene transfer
  • Inner ear
  • Supporting cells

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5. / Ballana, Ester; Wang, Jing; Venail, Frédéric; Estivill, Xavier P.; Puel, Jean Luc; Arbonès, Maria L.; Bosch, Assumpció.

In: Neuroscience Letters, Vol. 442, No. 2, 12.09.2008, p. 134-139.

Research output: Contribution to journalArticle

Ballana, Ester ; Wang, Jing ; Venail, Frédéric ; Estivill, Xavier P. ; Puel, Jean Luc ; Arbonès, Maria L. ; Bosch, Assumpció. / Efficient and specific transduction of cochlear supporting cells by adeno-associated virus serotype 5. In: Neuroscience Letters. 2008 ; Vol. 442, No. 2. pp. 134-139.
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