Effects of Systemically Administered Hydrocortisone on the Human Immunome

Matthew J. Olnes, Yuri Kotliarov, Angélique Biancotto, Foo Cheung, Jinguo Chen, Rongye Shi, Huizhi Zhou, Ena Wang, John S. Tsang, Robert Nussenblatt

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Corticosteroids have been used for decades to modulate inflammation therapeutically, yet there is a paucity of data on their effects in humans. We examined the changes in cellular and molecular immune system parameters, or "immunome", in healthy humans after systemic corticosteroid administration. We used multiplexed techniques to query the immunome in 20 volunteers at baseline, and after intravenous hydrocortisone (HC) administered at moderate (250 mg) and low (50 mg) doses, to provide insight into how corticosteroids exert their effects. We performed comprehensive phenotyping of 120 lymphocyte subsets by high dimensional flow cytometry, and observed a decline in circulating specific B and T cell subsets, which reached their nadir 4-8 hours after administration of HC. However, B and T cells rebounded above baseline 24 hours after HC infusion, while NK cell numbers remained stable. Whole transcriptome profiling revealed down regulation of NF-κB signaling, apoptosis, and cell death signaling transcripts that preceded lymphocyte population changes, with activation of NK cell and glucocorticoid receptor signaling transcripts. Our study is the first to systematically characterize the effects of corticosteroids on the human immunome, and we demonstrate that HC exerts differential effects on B and T lymphocytes and natural killer cells in humans.

Original languageEnglish
Article number23002
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 14 Mar 2016

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Hydrocortisone
Adrenal Cortex Hormones
Natural Killer Cells
B-Lymphocytes
Natural Killer Cell Receptors
B-Lymphocyte Subsets
T-Lymphocytes
Lymphocyte Subsets
Glucocorticoid Receptors
T-Lymphocyte Subsets
Gene Expression Profiling
Volunteers
Immune System
Flow Cytometry
Cell Death
Down-Regulation
Cell Count
Lymphocytes
Apoptosis
Inflammation

ASJC Scopus subject areas

  • General

Cite this

Olnes, M. J., Kotliarov, Y., Biancotto, A., Cheung, F., Chen, J., Shi, R., ... Nussenblatt, R. (2016). Effects of Systemically Administered Hydrocortisone on the Human Immunome. Scientific Reports, 6, [23002]. https://doi.org/10.1038/srep23002

Effects of Systemically Administered Hydrocortisone on the Human Immunome. / Olnes, Matthew J.; Kotliarov, Yuri; Biancotto, Angélique; Cheung, Foo; Chen, Jinguo; Shi, Rongye; Zhou, Huizhi; Wang, Ena; Tsang, John S.; Nussenblatt, Robert.

In: Scientific Reports, Vol. 6, 23002, 14.03.2016.

Research output: Contribution to journalArticle

Olnes, MJ, Kotliarov, Y, Biancotto, A, Cheung, F, Chen, J, Shi, R, Zhou, H, Wang, E, Tsang, JS & Nussenblatt, R 2016, 'Effects of Systemically Administered Hydrocortisone on the Human Immunome', Scientific Reports, vol. 6, 23002. https://doi.org/10.1038/srep23002
Olnes MJ, Kotliarov Y, Biancotto A, Cheung F, Chen J, Shi R et al. Effects of Systemically Administered Hydrocortisone on the Human Immunome. Scientific Reports. 2016 Mar 14;6. 23002. https://doi.org/10.1038/srep23002
Olnes, Matthew J. ; Kotliarov, Yuri ; Biancotto, Angélique ; Cheung, Foo ; Chen, Jinguo ; Shi, Rongye ; Zhou, Huizhi ; Wang, Ena ; Tsang, John S. ; Nussenblatt, Robert. / Effects of Systemically Administered Hydrocortisone on the Human Immunome. In: Scientific Reports. 2016 ; Vol. 6.
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