Effects of Hyperglycemia on Vascular Smooth Muscle Ca2+ Signaling

Nahed El-Najjar, Rashmi P. Kulkarni, Nancy Nader, Rawad Hodeify, Khaled Machaca

Research output: Contribution to journalArticle

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Abstract

Diabetes is a complex disease that is characterized with hyperglycemia, dyslipidemia, and insulin resistance. These pathologies are associated with significant cardiovascular implications that affect both the macro- and microvasculature. It is therefore important to understand the effects of various pathologies associated with diabetes on the vasculature. Here we directly test the effects of hyperglycemia on vascular smooth muscle (VSM) Ca2+ signaling in an isolated in vitro system using the A7r5 rat aortic cell line as a model. We find that prolonged exposure of A7r5 cells to hyperglycemia (weeks) is associated with changes to Ca2+ signaling, including most prominently an inhibition of the passive ER Ca2+ leak and the sarcoplasmic reticulum Ca2+-ATPase (SERCA). To translate these findings to the in vivo condition, we used primary VSM cells from normal and diabetic subjects and find that only the inhibition of the ER Ca2+ leaks replicates in cells from diabetic donors. These results show that prolonged hyperglycemia in isolation alters the Ca2+ signaling machinery in VSM cells. However, these alterations are not readily translatable to the whole organism situation where alterations to the Ca2+ signaling machinery are different.

Original languageEnglish
Article number3691349
JournalBioMed Research International
Volume2017
DOIs
Publication statusPublished - 21 Jun 2017

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Vascular Smooth Muscle
Hyperglycemia
Muscle
Pathology
Medical problems
Smooth Muscle Myocytes
Machinery
Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Dyslipidemias
Microvessels
Macros
Insulin Resistance
Rats
Cells
Insulin
Cell Line

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Effects of Hyperglycemia on Vascular Smooth Muscle Ca2+ Signaling. / El-Najjar, Nahed; Kulkarni, Rashmi P.; Nader, Nancy; Hodeify, Rawad; Machaca, Khaled.

In: BioMed Research International, Vol. 2017, 3691349, 21.06.2017.

Research output: Contribution to journalArticle

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