Effects of exogenous ganglioside and cholesterol application on excitability of aplysia neurons

Dietrich Busselberg, Martyn L. Evans, David O. Carpenter, Hinrich Rahmann

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effects of pressure-ejected gangliosides GM1 and GMix ("Cronassial" and cholesterol dissolved in sea water on the electrophysiological characteristics of Aplysia neurons were studied using voltage-clamp recording techniques. Two types of electrophysiological effects were found. In about 5% of neurons brief pulses (0.1-0.2 sec) of GM1 or GMix elicited fast and large currents associated with an increase in membrane conductance and clear reversal potentials. These currents were similar to those elicited by common neurotransmitters. Thus it appears that gangliosides may activate a membrane-bound receptor on at least some neurons. Most (about 85% of the 121 neurons studied showed responses to longer pulses (1.0-2.5 sec) of gangliosides. These responses were much smaller, usually had a relatively slow component, and could be mimicked by application of cholesterol. The currents elicited were either inward or outward and were often biphasic, with an small initial outward component followed by a larger slow inward current. The responses often became larger upon repeated application at short intervals, and long periods of wash were required for recovery. This type of response appears to reflect changes in the electrical properties of the cell induced by incorporation of small amounts of gangliosides or cholesterol into the membrane.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalMolecular Membrane Biology
Volume8
Issue number1
DOIs
Publication statusPublished - 1989
Externally publishedYes

Fingerprint

Aplysia
Gangliosides
Neurons
Cholesterol
Membranes
G(M1) Ganglioside
Clamping devices
Seawater
Patch-Clamp Techniques
Neurotransmitter Agents
Electric properties
Pressure
Recovery
Water
Electric potential

Keywords

  • Aplysia
  • Cholesterol
  • Gangliosides
  • Membrane currents
  • Neurons
  • Voltage clamp

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Cite this

Effects of exogenous ganglioside and cholesterol application on excitability of aplysia neurons. / Busselberg, Dietrich; Evans, Martyn L.; Carpenter, David O.; Rahmann, Hinrich.

In: Molecular Membrane Biology, Vol. 8, No. 1, 1989, p. 19-26.

Research output: Contribution to journalArticle

Busselberg, Dietrich ; Evans, Martyn L. ; Carpenter, David O. ; Rahmann, Hinrich. / Effects of exogenous ganglioside and cholesterol application on excitability of aplysia neurons. In: Molecular Membrane Biology. 1989 ; Vol. 8, No. 1. pp. 19-26.
@article{aed12f5014d04540ad03ece9c9704c32,
title = "Effects of exogenous ganglioside and cholesterol application on excitability of aplysia neurons",
abstract = "The effects of pressure-ejected gangliosides GM1 and GMix ({"}Cronassial{"} and cholesterol dissolved in sea water on the electrophysiological characteristics of Aplysia neurons were studied using voltage-clamp recording techniques. Two types of electrophysiological effects were found. In about 5{\%} of neurons brief pulses (0.1-0.2 sec) of GM1 or GMix elicited fast and large currents associated with an increase in membrane conductance and clear reversal potentials. These currents were similar to those elicited by common neurotransmitters. Thus it appears that gangliosides may activate a membrane-bound receptor on at least some neurons. Most (about 85{\%} of the 121 neurons studied showed responses to longer pulses (1.0-2.5 sec) of gangliosides. These responses were much smaller, usually had a relatively slow component, and could be mimicked by application of cholesterol. The currents elicited were either inward or outward and were often biphasic, with an small initial outward component followed by a larger slow inward current. The responses often became larger upon repeated application at short intervals, and long periods of wash were required for recovery. This type of response appears to reflect changes in the electrical properties of the cell induced by incorporation of small amounts of gangliosides or cholesterol into the membrane.",
keywords = "Aplysia, Cholesterol, Gangliosides, Membrane currents, Neurons, Voltage clamp",
author = "Dietrich Busselberg and Evans, {Martyn L.} and Carpenter, {David O.} and Hinrich Rahmann",
year = "1989",
doi = "10.3109/09687688909025823",
language = "English",
volume = "8",
pages = "19--26",
journal = "Molecular Membrane Biology",
issn = "0968-7688",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Effects of exogenous ganglioside and cholesterol application on excitability of aplysia neurons

AU - Busselberg, Dietrich

AU - Evans, Martyn L.

AU - Carpenter, David O.

AU - Rahmann, Hinrich

PY - 1989

Y1 - 1989

N2 - The effects of pressure-ejected gangliosides GM1 and GMix ("Cronassial" and cholesterol dissolved in sea water on the electrophysiological characteristics of Aplysia neurons were studied using voltage-clamp recording techniques. Two types of electrophysiological effects were found. In about 5% of neurons brief pulses (0.1-0.2 sec) of GM1 or GMix elicited fast and large currents associated with an increase in membrane conductance and clear reversal potentials. These currents were similar to those elicited by common neurotransmitters. Thus it appears that gangliosides may activate a membrane-bound receptor on at least some neurons. Most (about 85% of the 121 neurons studied showed responses to longer pulses (1.0-2.5 sec) of gangliosides. These responses were much smaller, usually had a relatively slow component, and could be mimicked by application of cholesterol. The currents elicited were either inward or outward and were often biphasic, with an small initial outward component followed by a larger slow inward current. The responses often became larger upon repeated application at short intervals, and long periods of wash were required for recovery. This type of response appears to reflect changes in the electrical properties of the cell induced by incorporation of small amounts of gangliosides or cholesterol into the membrane.

AB - The effects of pressure-ejected gangliosides GM1 and GMix ("Cronassial" and cholesterol dissolved in sea water on the electrophysiological characteristics of Aplysia neurons were studied using voltage-clamp recording techniques. Two types of electrophysiological effects were found. In about 5% of neurons brief pulses (0.1-0.2 sec) of GM1 or GMix elicited fast and large currents associated with an increase in membrane conductance and clear reversal potentials. These currents were similar to those elicited by common neurotransmitters. Thus it appears that gangliosides may activate a membrane-bound receptor on at least some neurons. Most (about 85% of the 121 neurons studied showed responses to longer pulses (1.0-2.5 sec) of gangliosides. These responses were much smaller, usually had a relatively slow component, and could be mimicked by application of cholesterol. The currents elicited were either inward or outward and were often biphasic, with an small initial outward component followed by a larger slow inward current. The responses often became larger upon repeated application at short intervals, and long periods of wash were required for recovery. This type of response appears to reflect changes in the electrical properties of the cell induced by incorporation of small amounts of gangliosides or cholesterol into the membrane.

KW - Aplysia

KW - Cholesterol

KW - Gangliosides

KW - Membrane currents

KW - Neurons

KW - Voltage clamp

UR - http://www.scopus.com/inward/record.url?scp=0024350760&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024350760&partnerID=8YFLogxK

U2 - 10.3109/09687688909025823

DO - 10.3109/09687688909025823

M3 - Article

VL - 8

SP - 19

EP - 26

JO - Molecular Membrane Biology

JF - Molecular Membrane Biology

SN - 0968-7688

IS - 1

ER -