Effects of dehydration on corneal tissue absorption of topical azithromycin in rabbits

Khalid F. Tabbara, Amgad A. Kotb, Ehab F. Hammouda, Naser Elkum

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: To assess the corneal tissue absorption of azithromycin in desiccated and normal eyes of rabbits. Methods: A total of 25 New Zealand Albino rabbits weighing 2-3 kg each were included. One eye of each rabbit was desiccated. The other eye was left as control. Azithromycin 1.5% topical eyedrops were instilled in both eyes. Five rabbits were sacrificed at each of the following time points: 30 min, 3 hr, 6 hr, and 12 hr. Corneal tissues specimens were subjected to high-performance liquid chromatography mass spectrometry. A paired t test was used to evaluate the statistical difference in corneal tissue absorptions of azithromycin at each time point. Results: The mean corneal tissue levels of azithromycin in dry eyes were 66.3 μg/ml, 92.6 μg/ml, 117.5 μg/ml, and 179.9 μg/ml, and the mean corneal tissue levels of azithromycin in normal eyes were 42.0 μg/ml, 43.4 μg/ml, 43.3 μg/ml, and 80.0 μg/ml at 30 min, 3 hr, 6 hr, and 12 hr respectively. Both groups showed increase in corneal tissue absorption overtime (p < 0.0001). Significantly higher levels of azithromycin were noted in dry eyes at each time point except at the 30-min time point. Conclusions: This study demonstrated that corneas exposed to desiccation showed statistically significant increase in azithromycin level compared to normal eyes.

Original languageEnglish
Pages (from-to)915-918
Number of pages4
JournalCurrent Eye Research
Volume30
Issue number10
DOIs
Publication statusPublished - 1 Oct 2005
Externally publishedYes

Fingerprint

Azithromycin
Dehydration
Rabbits
Desiccation
Ophthalmic Solutions
Cornea
Mass Spectrometry
High Pressure Liquid Chromatography

Keywords

  • Antibiotics
  • Azithromycin
  • Cornea
  • Dry eyes
  • Keratitis

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Effects of dehydration on corneal tissue absorption of topical azithromycin in rabbits. / Tabbara, Khalid F.; Kotb, Amgad A.; Hammouda, Ehab F.; Elkum, Naser.

In: Current Eye Research, Vol. 30, No. 10, 01.10.2005, p. 915-918.

Research output: Contribution to journalArticle

Tabbara, Khalid F. ; Kotb, Amgad A. ; Hammouda, Ehab F. ; Elkum, Naser. / Effects of dehydration on corneal tissue absorption of topical azithromycin in rabbits. In: Current Eye Research. 2005 ; Vol. 30, No. 10. pp. 915-918.
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