Effect of ovalbumin and anti-foam B on the survivability of spore forming bacteria collected in a Wetted Wall Cyclone

Asmaa S. Kassab, Maria D. King, Bing Guo, Andrew R. McFarland

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The effect of different collection fluids in maintaining the survivability, defined as the culturability in the archived sample divided by the culturability in the as-collected sample, were experimentally determined for Bacillus globigii (BG). The spores were collected using a 100 L/min Wetted Wall Cyclone (WWC) and archived for five days at 25°C and 4°C. Aqueous solutions of 0.01% Tween20 and 30% Ethylene glycol (EG), with or without 0.5% ovalbumin (OA), were used as collection fluids with 0.2% Antifoam B (A-F). The spores were more stable in EG and at 4°C, very likely due to the surfactant effect of the Tween20, which would remove the spore coat and initiate germination. Adding OA significantly increased the survivability, especially at RT while adding AF increased the survivability of BG in EG. The best collection fluid that can maintain the survivability of BG, during an archiving period of five days is EG + AF.

Original languageEnglish
Title of host publication12th International Conference on Indoor Air Quality and Climate 2011
Pages2866-2871
Number of pages6
Volume4
Publication statusPublished - 2011
Externally publishedYes
Event12th International Conference on Indoor Air Quality and Climate 2011 - Austin, TX, United States
Duration: 5 Jun 201110 Jun 2011

Other

Other12th International Conference on Indoor Air Quality and Climate 2011
CountryUnited States
CityAustin, TX
Period5/6/1110/6/11

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Keywords

  • Archiving
  • Bacillus globigii (BG)
  • Biosampler
  • Ethylene Glycol (EG)
  • Tween20 (TW20)

ASJC Scopus subject areas

  • Pollution

Cite this

Kassab, A. S., King, M. D., Guo, B., & McFarland, A. R. (2011). Effect of ovalbumin and anti-foam B on the survivability of spore forming bacteria collected in a Wetted Wall Cyclone. In 12th International Conference on Indoor Air Quality and Climate 2011 (Vol. 4, pp. 2866-2871)