Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells

Andrea Gojova, Jun Tae Lee, Heejung S. Jung, Bing Guo, Abdul I. Barakat, Ian M. Kennedy

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Because vascular endothelial cell inflammation is critical in the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response. To test the hypothesis, we incubated HAECs for 4h with different concentrations (0.00150μg/ml) of CeO2 nanoparticles and subsequently measured mRNA levels of the three inflammatory markers intercellular adhesion molecule 1 (ICAM-1), interleukin (IL)-8, and monocyte chemotactic protein (MCP-1) using real-time polymerase chain reaction (PCR). Ceria nanoparticles caused very little inflammatory response in HAECs, even at the highest dose. This material is apparently rather benign in comparison with Y2O3 and ZnO nanoparticles that we have studied previously. These results suggest that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends strongly on particle composition.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalInhalation Toxicology
Volume21
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Endothelial cells
Nanoparticles
Endothelial Cells
Inflammation
Monocyte Chemoattractant Proteins
Metal Nanoparticles
Chemokine CCL2
Polymerase chain reaction
Cerium compounds
Pathology
Intercellular Adhesion Molecule-1
Interleukin-8
Oxides
Real-Time Polymerase Chain Reaction
Metals
ceric oxide
Messenger RNA
Chemical analysis

Keywords

  • Ceria
  • Endothelial
  • Inflammation
  • Nanoparticles

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Gojova, A., Lee, J. T., Jung, H. S., Guo, B., Barakat, A. I., & Kennedy, I. M. (2009). Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells. Inhalation Toxicology, 21(SUPPL. 1), 123-130. https://doi.org/10.1080/08958370902942582

Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells. / Gojova, Andrea; Lee, Jun Tae; Jung, Heejung S.; Guo, Bing; Barakat, Abdul I.; Kennedy, Ian M.

In: Inhalation Toxicology, Vol. 21, No. SUPPL. 1, 2009, p. 123-130.

Research output: Contribution to journalArticle

Gojova, A, Lee, JT, Jung, HS, Guo, B, Barakat, AI & Kennedy, IM 2009, 'Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells', Inhalation Toxicology, vol. 21, no. SUPPL. 1, pp. 123-130. https://doi.org/10.1080/08958370902942582
Gojova, Andrea ; Lee, Jun Tae ; Jung, Heejung S. ; Guo, Bing ; Barakat, Abdul I. ; Kennedy, Ian M. / Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells. In: Inhalation Toxicology. 2009 ; Vol. 21, No. SUPPL. 1. pp. 123-130.
@article{ce3782f9a8654c9c874148cdfd22902d,
title = "Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells",
abstract = "Because vascular endothelial cell inflammation is critical in the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response. To test the hypothesis, we incubated HAECs for 4h with different concentrations (0.00150μg/ml) of CeO2 nanoparticles and subsequently measured mRNA levels of the three inflammatory markers intercellular adhesion molecule 1 (ICAM-1), interleukin (IL)-8, and monocyte chemotactic protein (MCP-1) using real-time polymerase chain reaction (PCR). Ceria nanoparticles caused very little inflammatory response in HAECs, even at the highest dose. This material is apparently rather benign in comparison with Y2O3 and ZnO nanoparticles that we have studied previously. These results suggest that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends strongly on particle composition.",
keywords = "Ceria, Endothelial, Inflammation, Nanoparticles",
author = "Andrea Gojova and Lee, {Jun Tae} and Jung, {Heejung S.} and Bing Guo and Barakat, {Abdul I.} and Kennedy, {Ian M.}",
year = "2009",
doi = "10.1080/08958370902942582",
language = "English",
volume = "21",
pages = "123--130",
journal = "Inhalation Toxicology",
issn = "0895-8378",
publisher = "Informa Healthcare",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Effect of cerium oxide nanoparticles on inflammation in vascular endothelial cells

AU - Gojova, Andrea

AU - Lee, Jun Tae

AU - Jung, Heejung S.

AU - Guo, Bing

AU - Barakat, Abdul I.

AU - Kennedy, Ian M.

PY - 2009

Y1 - 2009

N2 - Because vascular endothelial cell inflammation is critical in the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response. To test the hypothesis, we incubated HAECs for 4h with different concentrations (0.00150μg/ml) of CeO2 nanoparticles and subsequently measured mRNA levels of the three inflammatory markers intercellular adhesion molecule 1 (ICAM-1), interleukin (IL)-8, and monocyte chemotactic protein (MCP-1) using real-time polymerase chain reaction (PCR). Ceria nanoparticles caused very little inflammatory response in HAECs, even at the highest dose. This material is apparently rather benign in comparison with Y2O3 and ZnO nanoparticles that we have studied previously. These results suggest that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends strongly on particle composition.

AB - Because vascular endothelial cell inflammation is critical in the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response. To test the hypothesis, we incubated HAECs for 4h with different concentrations (0.00150μg/ml) of CeO2 nanoparticles and subsequently measured mRNA levels of the three inflammatory markers intercellular adhesion molecule 1 (ICAM-1), interleukin (IL)-8, and monocyte chemotactic protein (MCP-1) using real-time polymerase chain reaction (PCR). Ceria nanoparticles caused very little inflammatory response in HAECs, even at the highest dose. This material is apparently rather benign in comparison with Y2O3 and ZnO nanoparticles that we have studied previously. These results suggest that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends strongly on particle composition.

KW - Ceria

KW - Endothelial

KW - Inflammation

KW - Nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=70350700664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350700664&partnerID=8YFLogxK

U2 - 10.1080/08958370902942582

DO - 10.1080/08958370902942582

M3 - Article

VL - 21

SP - 123

EP - 130

JO - Inhalation Toxicology

JF - Inhalation Toxicology

SN - 0895-8378

IS - SUPPL. 1

ER -