Effect of calcium on nicotine-induced current expressed by an atypical α-bungarotoxin-insensitive nAChR2

Steeve H. Thany, Raphael Jean Courjaret, Bruno Lapied

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Two distinct native alpha-bungarotoxin (α-Bgt)-insensitive nicotinic acetylcholine receptors (nAChRs), named nAChR1 and nAChR2, were identified in the cockroach Periplaneta americana dorsal unpaired median (DUM) neurons. They differed in their electrophysiological, pharmacological properties and intracellular regulation pathways. nAChR2 being an atypical nicotinic receptor closed upon agonist application and its current-voltage relationship resulted from a reduction in potassium conductance. In this study, using whole-cell patch-clamp technique, we demonstrated that calcium modulated nAChR2-mediated nicotine response. Under 0.5 μM α-Bgt and 20 mM d-tubocurarine, the nicotine-induced inward current amplitude was strongly reduced in the presence of intracellularly applied BAPTA or bath application of calcium-free solution. In addition, using cadmium chloride, we showed that nicotine response was modulated by extracellular calcium through plasma membrane calcium channels. Moreover, extracellular application of caffeine and thapsigargin reduced nAChR2-mediated response. Together these experiments revealed a complex calcium-dependent regulation of nAChR2.

Original languageEnglish
Pages (from-to)317-321
Number of pages5
JournalNeuroscience Letters
Volume438
Issue number3
DOIs
Publication statusPublished - 27 Jun 2008
Externally publishedYes

Fingerprint

Bungarotoxins
Nicotine
Calcium
Nicotinic Receptors
Periplaneta
Cadmium Chloride
Tubocurarine
Cockroaches
Thapsigargin
Patch-Clamp Techniques
Calcium Channels
Caffeine
Baths
Ion Channels
Potassium
Cell Membrane
Pharmacology
Neurons

Keywords

  • Calcium
  • Cockroach
  • DUM neurons
  • Insect
  • Nicotinic receptors
  • Patch-clamp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effect of calcium on nicotine-induced current expressed by an atypical α-bungarotoxin-insensitive nAChR2. / Thany, Steeve H.; Courjaret, Raphael Jean; Lapied, Bruno.

In: Neuroscience Letters, Vol. 438, No. 3, 27.06.2008, p. 317-321.

Research output: Contribution to journalArticle

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