Ectopic expression of thyrotropin releasing hormone (TRH) receptors in liver modulates organ function to regulate blood glucose by TRH

Gerhard Wolff, Andrea Mastrangeli, Marcos Heinflink, Erik Falck-Pedersen, Marvin C. Gershengorn, Ronald Crystal

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Maintenance of blood glucose by the liver is normally initiated by extracellular regulatory molecules such as glucagon and vasopressin triggering specific hepatocyte receptors to activate the cAMP or phosphoinositide signal transduction pathways, respectively. We now show that the normal ligand-receptor regulators of blood glucose in the liver can be bypassed using an adenovirus vector expressing the mouse pituitary thyrotropin releasing hormone receptor (TRHR) cDNA ectopically in rat liver in vivo. The ectopically expressed TRHR links to the phosphoinositide pathway, providing a means to regulate liver function with TRH, an extracellular ligand that does not normally affect hepatic function. Administration of TRH to these animals activates the phosphoinositide pathway, resulting in a sustained rise in blood glucose. It should be possible to use this general strategy to modulate the differentiated functions of target organs in a wide variety of pathologic states.

Original languageEnglish
Pages (from-to)274-279
Number of pages6
JournalNature Genetics
Volume12
Issue number3
DOIs
Publication statusPublished - 1 Mar 1996
Externally publishedYes

Fingerprint

Thyrotropin Releasing Hormone Receptors
Thyrotropin-Releasing Hormone
Blood Glucose
Phosphatidylinositols
Liver
Ligands
Pituitary Hormones
Glucagon
Vasopressins
Adenoviridae
Hepatocytes
Signal Transduction
Complementary DNA
Maintenance
Ectopic Gene Expression

ASJC Scopus subject areas

  • Genetics

Cite this

Ectopic expression of thyrotropin releasing hormone (TRH) receptors in liver modulates organ function to regulate blood glucose by TRH. / Wolff, Gerhard; Mastrangeli, Andrea; Heinflink, Marcos; Falck-Pedersen, Erik; Gershengorn, Marvin C.; Crystal, Ronald.

In: Nature Genetics, Vol. 12, No. 3, 01.03.1996, p. 274-279.

Research output: Contribution to journalArticle

Wolff, Gerhard ; Mastrangeli, Andrea ; Heinflink, Marcos ; Falck-Pedersen, Erik ; Gershengorn, Marvin C. ; Crystal, Ronald. / Ectopic expression of thyrotropin releasing hormone (TRH) receptors in liver modulates organ function to regulate blood glucose by TRH. In: Nature Genetics. 1996 ; Vol. 12, No. 3. pp. 274-279.
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