Early implant healing: Promotion of platelet activation and cytokine release by topographical, chemical and biomimetical titanium surface modifications in vitro

P. W. Kämmerer, M. Gabriel, B. Al-Nawas, T. Scholz, C. M. Kirchmaier, M. O. Klein

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Objectives: Platelet releasate has been shown to promote osteogenetic cell proliferation and differentiation. Topography and chemistry of biomaterials have high impact on platelet activation. More specifically, the bioactive cell adhesive peptide sequence Arg-Gly-Asp (RGD) triggers platelet activation mediated by the α IIbβ 3 integrin receptor. Accordingly, topographical, chemical and biomimetical (immobilized RGD peptide) modifications of titanium (Ti) surfaces may enhance early platelet activation and bony healing of implants. Therefore, the aim of the study was to evaluate platelet activation with subsequent platelet-derived cytokine release by accordingly modified Ti surfaces. Materials and methods: Pre-treated (PT; mean roughness [R a]=0.04μm, contact angle [CA]=91°), acid-etched (A, R a=0.83μm, CA=106°), large grit-sandblasted, acid-etched (SLA, R a=3.2μm, CA=109°) as well as hydrophilically modified acid-etched (modA, R a=0.83μm, CA=0) and modified large grit-sandblasted, acid-etched (modSLA, R a=3.2μm; CA=0°) titanium surfaces were investigated. Additionally, RGD peptides were chemically immobilized on PT, A and SLA surfaces (PT-RGD [CA=18°], A-RGD [CA=0°], SLA-RGD [CA=0°]). The different Ti surfaces were incubated with platelet concentrate of three healthy volunteers at room temperature for 15min and for 30min. High thrombogenous collagen served as the control group. Out of the supernatant, platelet consumption was assessed via platelet count (PC). Cytokine release was quantified via the level of platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). Results:: After 15min, especially the rough SLA surface showed a strong decrease in PC and a strong increase in VEGF and PDGF levels. After 30min, high platelet consumption as well as high levels of VEGF and PDGF were measured for unspecifically modified (modA) and especially for biomimetic, specifically modified (PT-RGD, A-RGD) surfaces, indicating a delayed effect of the surface modifications on platelet activation. Discussion: Modifications of surface roughness modifications appear to influence early platelet activation and cytokine release after 15min whereas surface chemistry modifications with increased hydrophilic properties and surface modifications via RGD peptide on plainer surfaces lead to a further, more specific promotion of platelet activation and degranulation after 30min. The observed effect could be valuable for critical clinical situations like compromised bone sites.

Original languageEnglish
Pages (from-to)504-510
Number of pages7
JournalClinical Oral Implants Research
Volume23
Issue number4
DOIs
Publication statusPublished - Apr 2012
Externally publishedYes

Fingerprint

Platelet Activation
Titanium
Cytokines
Blood Platelets
Platelet-Derived Growth Factor
Vascular Endothelial Growth Factor A
Acids
Platelet Count
Biomimetics
Surface Properties
Biocompatible Materials
In Vitro Techniques
Integrins
Adhesives
Cell Differentiation
Healthy Volunteers
Collagen
Cell Proliferation
Bone and Bones
Control Groups

Keywords

  • PDGF
  • Platelet activation
  • RGD-peptide
  • Surface chemistry
  • Surface topography
  • Titanium implants
  • VEGF

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Early implant healing : Promotion of platelet activation and cytokine release by topographical, chemical and biomimetical titanium surface modifications in vitro. / Kämmerer, P. W.; Gabriel, M.; Al-Nawas, B.; Scholz, T.; Kirchmaier, C. M.; Klein, M. O.

In: Clinical Oral Implants Research, Vol. 23, No. 4, 04.2012, p. 504-510.

Research output: Contribution to journalArticle

Kämmerer, P. W. ; Gabriel, M. ; Al-Nawas, B. ; Scholz, T. ; Kirchmaier, C. M. ; Klein, M. O. / Early implant healing : Promotion of platelet activation and cytokine release by topographical, chemical and biomimetical titanium surface modifications in vitro. In: Clinical Oral Implants Research. 2012 ; Vol. 23, No. 4. pp. 504-510.
@article{249d30ea0de24a44b1d0e8dadd5c2b38,
title = "Early implant healing: Promotion of platelet activation and cytokine release by topographical, chemical and biomimetical titanium surface modifications in vitro",
abstract = "Objectives: Platelet releasate has been shown to promote osteogenetic cell proliferation and differentiation. Topography and chemistry of biomaterials have high impact on platelet activation. More specifically, the bioactive cell adhesive peptide sequence Arg-Gly-Asp (RGD) triggers platelet activation mediated by the α IIbβ 3 integrin receptor. Accordingly, topographical, chemical and biomimetical (immobilized RGD peptide) modifications of titanium (Ti) surfaces may enhance early platelet activation and bony healing of implants. Therefore, the aim of the study was to evaluate platelet activation with subsequent platelet-derived cytokine release by accordingly modified Ti surfaces. Materials and methods: Pre-treated (PT; mean roughness [R a]=0.04μm, contact angle [CA]=91°), acid-etched (A, R a=0.83μm, CA=106°), large grit-sandblasted, acid-etched (SLA, R a=3.2μm, CA=109°) as well as hydrophilically modified acid-etched (modA, R a=0.83μm, CA=0) and modified large grit-sandblasted, acid-etched (modSLA, R a=3.2μm; CA=0°) titanium surfaces were investigated. Additionally, RGD peptides were chemically immobilized on PT, A and SLA surfaces (PT-RGD [CA=18°], A-RGD [CA=0°], SLA-RGD [CA=0°]). The different Ti surfaces were incubated with platelet concentrate of three healthy volunteers at room temperature for 15min and for 30min. High thrombogenous collagen served as the control group. Out of the supernatant, platelet consumption was assessed via platelet count (PC). Cytokine release was quantified via the level of platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). Results:: After 15min, especially the rough SLA surface showed a strong decrease in PC and a strong increase in VEGF and PDGF levels. After 30min, high platelet consumption as well as high levels of VEGF and PDGF were measured for unspecifically modified (modA) and especially for biomimetic, specifically modified (PT-RGD, A-RGD) surfaces, indicating a delayed effect of the surface modifications on platelet activation. Discussion: Modifications of surface roughness modifications appear to influence early platelet activation and cytokine release after 15min whereas surface chemistry modifications with increased hydrophilic properties and surface modifications via RGD peptide on plainer surfaces lead to a further, more specific promotion of platelet activation and degranulation after 30min. The observed effect could be valuable for critical clinical situations like compromised bone sites.",
keywords = "PDGF, Platelet activation, RGD-peptide, Surface chemistry, Surface topography, Titanium implants, VEGF",
author = "K{\"a}mmerer, {P. W.} and M. Gabriel and B. Al-Nawas and T. Scholz and Kirchmaier, {C. M.} and Klein, {M. O.}",
year = "2012",
month = "4",
doi = "10.1111/j.1600-0501.2011.02153.x",
language = "English",
volume = "23",
pages = "504--510",
journal = "Clinical Oral Implants Research",
issn = "0905-7161",
publisher = "Blackwell Munksgaard",
number = "4",

}

TY - JOUR

T1 - Early implant healing

T2 - Promotion of platelet activation and cytokine release by topographical, chemical and biomimetical titanium surface modifications in vitro

AU - Kämmerer, P. W.

AU - Gabriel, M.

AU - Al-Nawas, B.

AU - Scholz, T.

AU - Kirchmaier, C. M.

AU - Klein, M. O.

PY - 2012/4

Y1 - 2012/4

N2 - Objectives: Platelet releasate has been shown to promote osteogenetic cell proliferation and differentiation. Topography and chemistry of biomaterials have high impact on platelet activation. More specifically, the bioactive cell adhesive peptide sequence Arg-Gly-Asp (RGD) triggers platelet activation mediated by the α IIbβ 3 integrin receptor. Accordingly, topographical, chemical and biomimetical (immobilized RGD peptide) modifications of titanium (Ti) surfaces may enhance early platelet activation and bony healing of implants. Therefore, the aim of the study was to evaluate platelet activation with subsequent platelet-derived cytokine release by accordingly modified Ti surfaces. Materials and methods: Pre-treated (PT; mean roughness [R a]=0.04μm, contact angle [CA]=91°), acid-etched (A, R a=0.83μm, CA=106°), large grit-sandblasted, acid-etched (SLA, R a=3.2μm, CA=109°) as well as hydrophilically modified acid-etched (modA, R a=0.83μm, CA=0) and modified large grit-sandblasted, acid-etched (modSLA, R a=3.2μm; CA=0°) titanium surfaces were investigated. Additionally, RGD peptides were chemically immobilized on PT, A and SLA surfaces (PT-RGD [CA=18°], A-RGD [CA=0°], SLA-RGD [CA=0°]). The different Ti surfaces were incubated with platelet concentrate of three healthy volunteers at room temperature for 15min and for 30min. High thrombogenous collagen served as the control group. Out of the supernatant, platelet consumption was assessed via platelet count (PC). Cytokine release was quantified via the level of platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). Results:: After 15min, especially the rough SLA surface showed a strong decrease in PC and a strong increase in VEGF and PDGF levels. After 30min, high platelet consumption as well as high levels of VEGF and PDGF were measured for unspecifically modified (modA) and especially for biomimetic, specifically modified (PT-RGD, A-RGD) surfaces, indicating a delayed effect of the surface modifications on platelet activation. Discussion: Modifications of surface roughness modifications appear to influence early platelet activation and cytokine release after 15min whereas surface chemistry modifications with increased hydrophilic properties and surface modifications via RGD peptide on plainer surfaces lead to a further, more specific promotion of platelet activation and degranulation after 30min. The observed effect could be valuable for critical clinical situations like compromised bone sites.

AB - Objectives: Platelet releasate has been shown to promote osteogenetic cell proliferation and differentiation. Topography and chemistry of biomaterials have high impact on platelet activation. More specifically, the bioactive cell adhesive peptide sequence Arg-Gly-Asp (RGD) triggers platelet activation mediated by the α IIbβ 3 integrin receptor. Accordingly, topographical, chemical and biomimetical (immobilized RGD peptide) modifications of titanium (Ti) surfaces may enhance early platelet activation and bony healing of implants. Therefore, the aim of the study was to evaluate platelet activation with subsequent platelet-derived cytokine release by accordingly modified Ti surfaces. Materials and methods: Pre-treated (PT; mean roughness [R a]=0.04μm, contact angle [CA]=91°), acid-etched (A, R a=0.83μm, CA=106°), large grit-sandblasted, acid-etched (SLA, R a=3.2μm, CA=109°) as well as hydrophilically modified acid-etched (modA, R a=0.83μm, CA=0) and modified large grit-sandblasted, acid-etched (modSLA, R a=3.2μm; CA=0°) titanium surfaces were investigated. Additionally, RGD peptides were chemically immobilized on PT, A and SLA surfaces (PT-RGD [CA=18°], A-RGD [CA=0°], SLA-RGD [CA=0°]). The different Ti surfaces were incubated with platelet concentrate of three healthy volunteers at room temperature for 15min and for 30min. High thrombogenous collagen served as the control group. Out of the supernatant, platelet consumption was assessed via platelet count (PC). Cytokine release was quantified via the level of platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). Results:: After 15min, especially the rough SLA surface showed a strong decrease in PC and a strong increase in VEGF and PDGF levels. After 30min, high platelet consumption as well as high levels of VEGF and PDGF were measured for unspecifically modified (modA) and especially for biomimetic, specifically modified (PT-RGD, A-RGD) surfaces, indicating a delayed effect of the surface modifications on platelet activation. Discussion: Modifications of surface roughness modifications appear to influence early platelet activation and cytokine release after 15min whereas surface chemistry modifications with increased hydrophilic properties and surface modifications via RGD peptide on plainer surfaces lead to a further, more specific promotion of platelet activation and degranulation after 30min. The observed effect could be valuable for critical clinical situations like compromised bone sites.

KW - PDGF

KW - Platelet activation

KW - RGD-peptide

KW - Surface chemistry

KW - Surface topography

KW - Titanium implants

KW - VEGF

UR - http://www.scopus.com/inward/record.url?scp=84858074727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84858074727&partnerID=8YFLogxK

U2 - 10.1111/j.1600-0501.2011.02153.x

DO - 10.1111/j.1600-0501.2011.02153.x

M3 - Article

C2 - 21435015

AN - SCOPUS:84858074727

VL - 23

SP - 504

EP - 510

JO - Clinical Oral Implants Research

JF - Clinical Oral Implants Research

SN - 0905-7161

IS - 4

ER -