Early corneal cellular and nerve fiber pathology in young patients with type 1 diabetes mellitus identified using corneal confocal microscopy

Eszter Szalai, Eszter Deák, László Módis, Gábor Németh, András Berta, Annamária Nagy, Enikő Felszeghy, Rita Káposzta, Rayaz Malik, Adrienne Csutak

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

PURPOSE. The aim of this study was to quantify epithelial, stromal, and endothelial cell density, and subbasal nerve morphology in young patients with type 1 diabetes mellitus with and without diabetic retinopathy. METHODS. A total of 28 young patients (mean age, 22.86 ± 9.05 years) with type 1 diabetes, with (n = 18) and without (n = 10) retinopathy, and 17 age-matched healthy control subjects (mean age, 26.53 ± 2.43 years) underwent corneal confocal microscopy (CCM). RESULTS. We found significantly lower epithelial (P < 0.0001) and endothelial (P = 0.001) cell densities and higher keratocyte cell density (P = 0.024) in patients with type 1 diabetes compared to controls. Significantly lower corneal nerve fiber density (P = 0.004), nerve branch density (P = 0.004), total nerve branch density (P = 0.04), and nerve fiber length (P = 0.001), and greater nerve fiber width (P = 0.04) were observed in patients with type 1 diabetes compared to control subjects. Significantly lower epithelial (P < 0.001) and endothelial (P = 0.02) cell densities, nerve branch density (P = 0.02), and nerve fiber length (P = 0.04), and significantly higher keratocyte cell density (P = 0.02) were found in patients with type 1 diabetes without retinopathy compared to control subjects. CONCLUSIONS. Corneal confocal microscopy identifies corneal cellular and small nerve fiber pathology in young patients with type 1 diabetes without retinopathy, which increases in severity in those with retinopathy. Corneal confocal microscopy appears to have considerable use as an imaging biomarker for early subclinical pathology in young patients with type 1 diabetes mellitus.

Original languageEnglish
Pages (from-to)853-858
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume57
Issue number3
DOIs
Publication statusPublished - 1 Mar 2016

Fingerprint

Type 1 Diabetes Mellitus
Nerve Fibers
Confocal Microscopy
Pathology
Cell Count
Diabetic Retinopathy
Stromal Cells
Healthy Volunteers
Endothelial Cells
Biomarkers
Epithelial Cells

Keywords

  • Diabetic neuropathy
  • Diabetic retinopathy
  • In vivo confocal microscopy
  • Subbasal corneal nerves
  • Type 1 diabetes mellitus

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Early corneal cellular and nerve fiber pathology in young patients with type 1 diabetes mellitus identified using corneal confocal microscopy. / Szalai, Eszter; Deák, Eszter; Módis, László; Németh, Gábor; Berta, András; Nagy, Annamária; Felszeghy, Enikő; Káposzta, Rita; Malik, Rayaz; Csutak, Adrienne.

In: Investigative Ophthalmology and Visual Science, Vol. 57, No. 3, 01.03.2016, p. 853-858.

Research output: Contribution to journalArticle

Szalai, Eszter ; Deák, Eszter ; Módis, László ; Németh, Gábor ; Berta, András ; Nagy, Annamária ; Felszeghy, Enikő ; Káposzta, Rita ; Malik, Rayaz ; Csutak, Adrienne. / Early corneal cellular and nerve fiber pathology in young patients with type 1 diabetes mellitus identified using corneal confocal microscopy. In: Investigative Ophthalmology and Visual Science. 2016 ; Vol. 57, No. 3. pp. 853-858.
@article{4c46dd5d08154416ba8ee733352c6e9c,
title = "Early corneal cellular and nerve fiber pathology in young patients with type 1 diabetes mellitus identified using corneal confocal microscopy",
abstract = "PURPOSE. The aim of this study was to quantify epithelial, stromal, and endothelial cell density, and subbasal nerve morphology in young patients with type 1 diabetes mellitus with and without diabetic retinopathy. METHODS. A total of 28 young patients (mean age, 22.86 ± 9.05 years) with type 1 diabetes, with (n = 18) and without (n = 10) retinopathy, and 17 age-matched healthy control subjects (mean age, 26.53 ± 2.43 years) underwent corneal confocal microscopy (CCM). RESULTS. We found significantly lower epithelial (P < 0.0001) and endothelial (P = 0.001) cell densities and higher keratocyte cell density (P = 0.024) in patients with type 1 diabetes compared to controls. Significantly lower corneal nerve fiber density (P = 0.004), nerve branch density (P = 0.004), total nerve branch density (P = 0.04), and nerve fiber length (P = 0.001), and greater nerve fiber width (P = 0.04) were observed in patients with type 1 diabetes compared to control subjects. Significantly lower epithelial (P < 0.001) and endothelial (P = 0.02) cell densities, nerve branch density (P = 0.02), and nerve fiber length (P = 0.04), and significantly higher keratocyte cell density (P = 0.02) were found in patients with type 1 diabetes without retinopathy compared to control subjects. CONCLUSIONS. Corneal confocal microscopy identifies corneal cellular and small nerve fiber pathology in young patients with type 1 diabetes without retinopathy, which increases in severity in those with retinopathy. Corneal confocal microscopy appears to have considerable use as an imaging biomarker for early subclinical pathology in young patients with type 1 diabetes mellitus.",
keywords = "Diabetic neuropathy, Diabetic retinopathy, In vivo confocal microscopy, Subbasal corneal nerves, Type 1 diabetes mellitus",
author = "Eszter Szalai and Eszter De{\'a}k and L{\'a}szl{\'o} M{\'o}dis and G{\'a}bor N{\'e}meth and Andr{\'a}s Berta and Annam{\'a}ria Nagy and Enikő Felszeghy and Rita K{\'a}poszta and Rayaz Malik and Adrienne Csutak",
year = "2016",
month = "3",
day = "1",
doi = "10.1167/iovs.15-18735",
language = "English",
volume = "57",
pages = "853--858",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "3",

}

TY - JOUR

T1 - Early corneal cellular and nerve fiber pathology in young patients with type 1 diabetes mellitus identified using corneal confocal microscopy

AU - Szalai, Eszter

AU - Deák, Eszter

AU - Módis, László

AU - Németh, Gábor

AU - Berta, András

AU - Nagy, Annamária

AU - Felszeghy, Enikő

AU - Káposzta, Rita

AU - Malik, Rayaz

AU - Csutak, Adrienne

PY - 2016/3/1

Y1 - 2016/3/1

N2 - PURPOSE. The aim of this study was to quantify epithelial, stromal, and endothelial cell density, and subbasal nerve morphology in young patients with type 1 diabetes mellitus with and without diabetic retinopathy. METHODS. A total of 28 young patients (mean age, 22.86 ± 9.05 years) with type 1 diabetes, with (n = 18) and without (n = 10) retinopathy, and 17 age-matched healthy control subjects (mean age, 26.53 ± 2.43 years) underwent corneal confocal microscopy (CCM). RESULTS. We found significantly lower epithelial (P < 0.0001) and endothelial (P = 0.001) cell densities and higher keratocyte cell density (P = 0.024) in patients with type 1 diabetes compared to controls. Significantly lower corneal nerve fiber density (P = 0.004), nerve branch density (P = 0.004), total nerve branch density (P = 0.04), and nerve fiber length (P = 0.001), and greater nerve fiber width (P = 0.04) were observed in patients with type 1 diabetes compared to control subjects. Significantly lower epithelial (P < 0.001) and endothelial (P = 0.02) cell densities, nerve branch density (P = 0.02), and nerve fiber length (P = 0.04), and significantly higher keratocyte cell density (P = 0.02) were found in patients with type 1 diabetes without retinopathy compared to control subjects. CONCLUSIONS. Corneal confocal microscopy identifies corneal cellular and small nerve fiber pathology in young patients with type 1 diabetes without retinopathy, which increases in severity in those with retinopathy. Corneal confocal microscopy appears to have considerable use as an imaging biomarker for early subclinical pathology in young patients with type 1 diabetes mellitus.

AB - PURPOSE. The aim of this study was to quantify epithelial, stromal, and endothelial cell density, and subbasal nerve morphology in young patients with type 1 diabetes mellitus with and without diabetic retinopathy. METHODS. A total of 28 young patients (mean age, 22.86 ± 9.05 years) with type 1 diabetes, with (n = 18) and without (n = 10) retinopathy, and 17 age-matched healthy control subjects (mean age, 26.53 ± 2.43 years) underwent corneal confocal microscopy (CCM). RESULTS. We found significantly lower epithelial (P < 0.0001) and endothelial (P = 0.001) cell densities and higher keratocyte cell density (P = 0.024) in patients with type 1 diabetes compared to controls. Significantly lower corneal nerve fiber density (P = 0.004), nerve branch density (P = 0.004), total nerve branch density (P = 0.04), and nerve fiber length (P = 0.001), and greater nerve fiber width (P = 0.04) were observed in patients with type 1 diabetes compared to control subjects. Significantly lower epithelial (P < 0.001) and endothelial (P = 0.02) cell densities, nerve branch density (P = 0.02), and nerve fiber length (P = 0.04), and significantly higher keratocyte cell density (P = 0.02) were found in patients with type 1 diabetes without retinopathy compared to control subjects. CONCLUSIONS. Corneal confocal microscopy identifies corneal cellular and small nerve fiber pathology in young patients with type 1 diabetes without retinopathy, which increases in severity in those with retinopathy. Corneal confocal microscopy appears to have considerable use as an imaging biomarker for early subclinical pathology in young patients with type 1 diabetes mellitus.

KW - Diabetic neuropathy

KW - Diabetic retinopathy

KW - In vivo confocal microscopy

KW - Subbasal corneal nerves

KW - Type 1 diabetes mellitus

UR - http://www.scopus.com/inward/record.url?scp=84960193510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960193510&partnerID=8YFLogxK

U2 - 10.1167/iovs.15-18735

DO - 10.1167/iovs.15-18735

M3 - Article

VL - 57

SP - 853

EP - 858

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 3

ER -