DYRK1A-Dosage Imbalance Perturbs NRSF/REST Levels, Deregulating Pluripotency and Embryonic Stem Cell Fate in Down Syndrome

Claudia Canzonetta, Claire Mulligan, Samuel Deutsch, Sandra Ruf, Aideen O'Doherty, Robert Lyle, Christelle Borel, Nathalie Lin-Marq, Frederic Delom, Jürgen Groet, Felix Schnappauf, Serena De Vita, Sharon Averill, John V. Priestley, Joanne E. Martin, Janet Shipley, Gareth Denyer, Charles J. Epstein, Cristina Fillat, Xavier P. Estivill & 4 others Victor L J Tybulewicz, Elizabeth M C Fisher, Stylianos E. Antonarakis, Dean Nizetic

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Down syndrome (DS) is the most common cause of mental retardation. Many neural phenotypes are shared between DS individuals and DS mouse models; however, the common underlying molecular pathogenetic mechanisms remain unclear. Using a transchromosomic model of DS, we show that a 30%-60% reduced expression of Nrsf/Rest (a key regulator of pluripotency and neuronal differentiation) is an alteration that persists in trisomy 21 from undifferentiated embryonic stem (ES) cells to adult brain and is reproducible across several DS models. Using partially trisomic ES cells, we map this effect to a three-gene segment of HSA21, containing DYRK1A. We independently identify the same locus as the most significant eQTL controlling REST expression in the human genome. We show that specifically silencing the third copy of DYRK1A rescues Rest levels, and we demonstrate altered Rest expression in response to inhibition of DYRK1A expression or kinase activity, and in a transgenic Dyrk1A mouse. We reveal that undifferentiated trisomy 21 ES cells show DYRK1A-dose-sensitive reductions in levels of some pluripotency regulators, causing premature expression of transcription factors driving early endodermal and mesodermal differentiation, partially overlapping recently reported downstream effects of Rest +/-. They produce embryoid bodies with elevated levels of the primitive endoderm progenitor marker Gata4 and a strongly reduced neuroectodermal progenitor compartment. Our results suggest that DYRK1A-mediated deregulation of REST is a very early pathological consequence of trisomy 21 with potential to disturb the development of all embryonic lineages, warranting closer research into its contribution to DS pathology and new rationales for therapeutic approaches.

Original languageEnglish
Pages (from-to)388-400
Number of pages13
JournalAmerican Journal of Human Genetics
Volume83
Issue number3
DOIs
Publication statusPublished - 12 Sep 2008
Externally publishedYes

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Embryonic Stem Cells
Down Syndrome
Embryoid Bodies
Endoderm
Human Genome
Intellectual Disability
Transgenic Mice
Embryonic Development
Transcription Factors
Phosphotransferases
Pathology
Phenotype
Brain
Research

ASJC Scopus subject areas

  • Genetics

Cite this

DYRK1A-Dosage Imbalance Perturbs NRSF/REST Levels, Deregulating Pluripotency and Embryonic Stem Cell Fate in Down Syndrome. / Canzonetta, Claudia; Mulligan, Claire; Deutsch, Samuel; Ruf, Sandra; O'Doherty, Aideen; Lyle, Robert; Borel, Christelle; Lin-Marq, Nathalie; Delom, Frederic; Groet, Jürgen; Schnappauf, Felix; De Vita, Serena; Averill, Sharon; Priestley, John V.; Martin, Joanne E.; Shipley, Janet; Denyer, Gareth; Epstein, Charles J.; Fillat, Cristina; Estivill, Xavier P.; Tybulewicz, Victor L J; Fisher, Elizabeth M C; Antonarakis, Stylianos E.; Nizetic, Dean.

In: American Journal of Human Genetics, Vol. 83, No. 3, 12.09.2008, p. 388-400.

Research output: Contribution to journalArticle

Canzonetta, C, Mulligan, C, Deutsch, S, Ruf, S, O'Doherty, A, Lyle, R, Borel, C, Lin-Marq, N, Delom, F, Groet, J, Schnappauf, F, De Vita, S, Averill, S, Priestley, JV, Martin, JE, Shipley, J, Denyer, G, Epstein, CJ, Fillat, C, Estivill, XP, Tybulewicz, VLJ, Fisher, EMC, Antonarakis, SE & Nizetic, D 2008, 'DYRK1A-Dosage Imbalance Perturbs NRSF/REST Levels, Deregulating Pluripotency and Embryonic Stem Cell Fate in Down Syndrome', American Journal of Human Genetics, vol. 83, no. 3, pp. 388-400. https://doi.org/10.1016/j.ajhg.2008.08.012
Canzonetta, Claudia ; Mulligan, Claire ; Deutsch, Samuel ; Ruf, Sandra ; O'Doherty, Aideen ; Lyle, Robert ; Borel, Christelle ; Lin-Marq, Nathalie ; Delom, Frederic ; Groet, Jürgen ; Schnappauf, Felix ; De Vita, Serena ; Averill, Sharon ; Priestley, John V. ; Martin, Joanne E. ; Shipley, Janet ; Denyer, Gareth ; Epstein, Charles J. ; Fillat, Cristina ; Estivill, Xavier P. ; Tybulewicz, Victor L J ; Fisher, Elizabeth M C ; Antonarakis, Stylianos E. ; Nizetic, Dean. / DYRK1A-Dosage Imbalance Perturbs NRSF/REST Levels, Deregulating Pluripotency and Embryonic Stem Cell Fate in Down Syndrome. In: American Journal of Human Genetics. 2008 ; Vol. 83, No. 3. pp. 388-400.
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AU - Canzonetta, Claudia

AU - Mulligan, Claire

AU - Deutsch, Samuel

AU - Ruf, Sandra

AU - O'Doherty, Aideen

AU - Lyle, Robert

AU - Borel, Christelle

AU - Lin-Marq, Nathalie

AU - Delom, Frederic

AU - Groet, Jürgen

AU - Schnappauf, Felix

AU - De Vita, Serena

AU - Averill, Sharon

AU - Priestley, John V.

AU - Martin, Joanne E.

AU - Shipley, Janet

AU - Denyer, Gareth

AU - Epstein, Charles J.

AU - Fillat, Cristina

AU - Estivill, Xavier P.

AU - Tybulewicz, Victor L J

AU - Fisher, Elizabeth M C

AU - Antonarakis, Stylianos E.

AU - Nizetic, Dean

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N2 - Down syndrome (DS) is the most common cause of mental retardation. Many neural phenotypes are shared between DS individuals and DS mouse models; however, the common underlying molecular pathogenetic mechanisms remain unclear. Using a transchromosomic model of DS, we show that a 30%-60% reduced expression of Nrsf/Rest (a key regulator of pluripotency and neuronal differentiation) is an alteration that persists in trisomy 21 from undifferentiated embryonic stem (ES) cells to adult brain and is reproducible across several DS models. Using partially trisomic ES cells, we map this effect to a three-gene segment of HSA21, containing DYRK1A. We independently identify the same locus as the most significant eQTL controlling REST expression in the human genome. We show that specifically silencing the third copy of DYRK1A rescues Rest levels, and we demonstrate altered Rest expression in response to inhibition of DYRK1A expression or kinase activity, and in a transgenic Dyrk1A mouse. We reveal that undifferentiated trisomy 21 ES cells show DYRK1A-dose-sensitive reductions in levels of some pluripotency regulators, causing premature expression of transcription factors driving early endodermal and mesodermal differentiation, partially overlapping recently reported downstream effects of Rest +/-. They produce embryoid bodies with elevated levels of the primitive endoderm progenitor marker Gata4 and a strongly reduced neuroectodermal progenitor compartment. Our results suggest that DYRK1A-mediated deregulation of REST is a very early pathological consequence of trisomy 21 with potential to disturb the development of all embryonic lineages, warranting closer research into its contribution to DS pathology and new rationales for therapeutic approaches.

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