Dual functions of largest NURF subunit NURF301 in nucleosome sliding and transcription factor interactions

Hua Xiao, Raphael Sandaltzopoulos, Hih Min Wang, Ali Hamiche, Ryan Ranallo, Kyu Min Lee, Dragony Fu, Carl Wu

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

NURF is an ISWI complex of four proteins that uses the energy of ATP hydrolysis to catalyze nucleosome sliding. Three NURF components have been identified previously. We have cloned cDNA encoding the largest NURF subunit, revealing a 301 kDa polypeptide (NURF301) that shares structural motifs with ACF1. We have reconstituted full and partial NURF complexes from recombinant proteins and show that NURF301 and the ISWI ATPase are necessary and sufficient for accurate and efficient nucleosome sliding. An HMGA/HMGI(Y)-like domain of NURF301 that facilitates nucleosome sliding indicates the importance of DNA conformational changes in the sliding mechanism. NURF301 also shows interactions with sequence-specific transcription factors, providing a basis for targeted recruitment of the NURF complex to specific genes.

Original languageEnglish
Pages (from-to)531-543
Number of pages13
JournalMolecular Cell
Volume8
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Nucleosomes
Transcription Factors
HMGA Proteins
Recombinant Proteins
Adenosine Triphosphatases
Hydrolysis
Complementary DNA
Adenosine Triphosphate
Peptides
DNA
Genes
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Dual functions of largest NURF subunit NURF301 in nucleosome sliding and transcription factor interactions. / Xiao, Hua; Sandaltzopoulos, Raphael; Wang, Hih Min; Hamiche, Ali; Ranallo, Ryan; Lee, Kyu Min; Fu, Dragony; Wu, Carl.

In: Molecular Cell, Vol. 8, No. 3, 2001, p. 531-543.

Research output: Contribution to journalArticle

Xiao, Hua ; Sandaltzopoulos, Raphael ; Wang, Hih Min ; Hamiche, Ali ; Ranallo, Ryan ; Lee, Kyu Min ; Fu, Dragony ; Wu, Carl. / Dual functions of largest NURF subunit NURF301 in nucleosome sliding and transcription factor interactions. In: Molecular Cell. 2001 ; Vol. 8, No. 3. pp. 531-543.
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AU - Fu, Dragony

AU - Wu, Carl

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