Dss1 regulates interaction of Brh2 with DNA

Qingwen Zhou, Nayef Mazloum, Ninghui Mao, Milorad Kojic, William K. Holloman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Brh2, the BRCA2 homologue in Ustilago maydis, plays a crucial role in homologous recombination by controlling Rad51. In turn, Brh2 is governed by Dss1, an intrinsically disordered protein that forms a tight complex with the C-terminal region of Brh2. This region of the protein associating with Dss1 is highly conserved in sequence and by comparison with mammalian BRCA2 corresponds to a part of the DNA binding domain with characteristic OB folds. The N-terminal region of Brh2 harbors a less-defined but powerful DNA binding site, the activity of which is revealed upon deletion of the C-terminal region. Full-length Brh2 complexed with Dss1 binds DNA slowly, while the N-terminal fragment binds quickly. The DNA binding activity of full-length Brh2 appears to correlate with dissociation of Dss1. Addition of Dss1 to the heterotypic Brh2-Dss1 complex attenuates DNA binding activity, but not by direct competition for the N-terminal DNA binding site. Conversely, the Brh2-Dss1 complex dissociates more quickly when DNA is present. These findings suggest a model in which binding of Brh2 toDNA is subject to allosteric regulation by Dss1.

Original languageEnglish
Pages (from-to)11929-11938
Number of pages10
JournalBiochemistry
Volume48
Issue number50
DOIs
Publication statusPublished - 22 Dec 2009
Externally publishedYes

Fingerprint

DNA
Binding Sites
Ustilago
Intrinsically Disordered Proteins
Allosteric Regulation
Conserved Sequence
Homologous Recombination
Ports and harbors
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Zhou, Q., Mazloum, N., Mao, N., Kojic, M., & Holloman, W. K. (2009). Dss1 regulates interaction of Brh2 with DNA. Biochemistry, 48(50), 11929-11938. https://doi.org/10.1021/bi901775j

Dss1 regulates interaction of Brh2 with DNA. / Zhou, Qingwen; Mazloum, Nayef; Mao, Ninghui; Kojic, Milorad; Holloman, William K.

In: Biochemistry, Vol. 48, No. 50, 22.12.2009, p. 11929-11938.

Research output: Contribution to journalArticle

Zhou, Q, Mazloum, N, Mao, N, Kojic, M & Holloman, WK 2009, 'Dss1 regulates interaction of Brh2 with DNA', Biochemistry, vol. 48, no. 50, pp. 11929-11938. https://doi.org/10.1021/bi901775j
Zhou Q, Mazloum N, Mao N, Kojic M, Holloman WK. Dss1 regulates interaction of Brh2 with DNA. Biochemistry. 2009 Dec 22;48(50):11929-11938. https://doi.org/10.1021/bi901775j
Zhou, Qingwen ; Mazloum, Nayef ; Mao, Ninghui ; Kojic, Milorad ; Holloman, William K. / Dss1 regulates interaction of Brh2 with DNA. In: Biochemistry. 2009 ; Vol. 48, No. 50. pp. 11929-11938.
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