Dss1 interaction with Brh2 as a regulatory mechanism for recombinational repair

Qingwen Zhou, Milorad Kojic, Zhimin Cao, Michael Lisby, Nayef Mazloum, William K. Holloman

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Brh2, the BRCA2 ortholog in Ustilago maydis, enables recombinational repair of DNA by controlling Rad51 and is in turn regulated by Dss1. Interplay with Rad51 is conducted via the BRC element located in the N-terminal region of the protein and through an unrelated domain, CRE, at the C terminus. Mutation in either BRC or CRE severely reduces functional activity, but repair deficiency of the brh2 mutant can be complemented by expressing BRC and CRE on different molecules. This intermolecular complementation is dependent upon the presence of Dss1. Brh2 molecules associate through the region overlapping with the Dss1-interacting domain to form at least dimer-sized complexes, which in turn, can be dissociated by Dss1 to monomer. We propose that cooperation between BRC and CRE domains and the Dss1-provoked dissociation of Brh2 complexes are requisite features of Brh2's molecular mechanism.

Original languageEnglish
Pages (from-to)2512-2526
Number of pages15
JournalMolecular and Cellular Biology
Volume27
Issue number7
DOIs
Publication statusPublished - Apr 2007
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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