Abstract
We have demonstrated that DSCR1 acts as a negative regulator of calcineurin-mediated signaling and that its transcript is overexpressed in the Down syndrome (DS) fetal brain. To evaluate the possible involvement of DSCR1 in DS, we have cloned the mouse gene and analyzed its expression pattern in the central nervous system (CNS). Early expression of Dscr1 is detected mainly in the heart tube and in the CNS in rhombomere 4 and the pretectum. From embryonic day 14.5 onwards, Dscr1 is widely distributed in the CNS but becomes more restricted as the brain matures. We confirmed its neuronal expression pattern in the adult, preferentially in Purkinje and pyramidal cells, by double labeling with glial fibrillary acidic protein. We also show that although Dscr1 is present in trisomy in the Ts65Dn mouse, the adult brain expression pattern is not significantly altered. This expression pattern indicated that Dscr1 is a developmentally regulated gene involved in neurogenesis and cardiogenesis and suggests that it may contribute to the alterations observed in these organ systems in DS patients.
| Original language | English |
|---|---|
| Pages (from-to) | 289-292 |
| Number of pages | 4 |
| Journal | Mechanisms of Development |
| Volume | 101 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- Calcineurin
- Chromosome 21
- Down syndrome
- Dscr1
- Heart
- Nervous system
- Nuclear factor of activated T cells
- Rhombomere
- Ts65Dn mouse
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
Dscr1, a novel endogenous inhibitor of calcineurin signaling, is expressed in the primitive ventricle of the heart and during neurogenesis. / Casas, C.; Martínez, S.; Pritchard, M. A.; Fuentes, J. J.; Nadal, M.; Guimerà, J.; Arbonés, M.; Flórez, J.; Soriano, E.; Estivill, Xavier P.; Alcántara, S.
In: Mechanisms of Development, Vol. 101, No. 1-2, 2001, p. 289-292.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dscr1, a novel endogenous inhibitor of calcineurin signaling, is expressed in the primitive ventricle of the heart and during neurogenesis
AU - Casas, C.
AU - Martínez, S.
AU - Pritchard, M. A.
AU - Fuentes, J. J.
AU - Nadal, M.
AU - Guimerà, J.
AU - Arbonés, M.
AU - Flórez, J.
AU - Soriano, E.
AU - Estivill, Xavier P.
AU - Alcántara, S.
PY - 2001
Y1 - 2001
N2 - We have demonstrated that DSCR1 acts as a negative regulator of calcineurin-mediated signaling and that its transcript is overexpressed in the Down syndrome (DS) fetal brain. To evaluate the possible involvement of DSCR1 in DS, we have cloned the mouse gene and analyzed its expression pattern in the central nervous system (CNS). Early expression of Dscr1 is detected mainly in the heart tube and in the CNS in rhombomere 4 and the pretectum. From embryonic day 14.5 onwards, Dscr1 is widely distributed in the CNS but becomes more restricted as the brain matures. We confirmed its neuronal expression pattern in the adult, preferentially in Purkinje and pyramidal cells, by double labeling with glial fibrillary acidic protein. We also show that although Dscr1 is present in trisomy in the Ts65Dn mouse, the adult brain expression pattern is not significantly altered. This expression pattern indicated that Dscr1 is a developmentally regulated gene involved in neurogenesis and cardiogenesis and suggests that it may contribute to the alterations observed in these organ systems in DS patients.
AB - We have demonstrated that DSCR1 acts as a negative regulator of calcineurin-mediated signaling and that its transcript is overexpressed in the Down syndrome (DS) fetal brain. To evaluate the possible involvement of DSCR1 in DS, we have cloned the mouse gene and analyzed its expression pattern in the central nervous system (CNS). Early expression of Dscr1 is detected mainly in the heart tube and in the CNS in rhombomere 4 and the pretectum. From embryonic day 14.5 onwards, Dscr1 is widely distributed in the CNS but becomes more restricted as the brain matures. We confirmed its neuronal expression pattern in the adult, preferentially in Purkinje and pyramidal cells, by double labeling with glial fibrillary acidic protein. We also show that although Dscr1 is present in trisomy in the Ts65Dn mouse, the adult brain expression pattern is not significantly altered. This expression pattern indicated that Dscr1 is a developmentally regulated gene involved in neurogenesis and cardiogenesis and suggests that it may contribute to the alterations observed in these organ systems in DS patients.
KW - Calcineurin
KW - Chromosome 21
KW - Down syndrome
KW - Dscr1
KW - Heart
KW - Nervous system
KW - Nuclear factor of activated T cells
KW - Rhombomere
KW - Ts65Dn mouse
UR - http://www.scopus.com/inward/record.url?scp=0035111345&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035111345&partnerID=8YFLogxK
U2 - 10.1016/S0925-4773(00)00583-9
DO - 10.1016/S0925-4773(00)00583-9
M3 - Article
VL - 101
SP - 289
EP - 292
JO - Mechanisms of Development
JF - Mechanisms of Development
SN - 0925-4773
IS - 1-2
ER -