Draft genome of neurotropic nematode parasite Angiostrongylus cantonensis, causative agent of human eosinophilic meningitis

Hoi Sen Yong, Praphathip Eamsobhana, Phaik Eem Lim, Rozaimi Mohamad Razali, Farhanah Abdul Aziz, Nurul Shielawati Mohamed Rosli, Johan Poole-Johnson, Arif Anwar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Angiostrongylus cantonensis is a bursate nematode parasite that causes eosinophilic meningitis (or meningoencephalitis) in humans in many parts of the world. The genomic data from A. cantonensis will form a useful resource for comparative genomic and chemogenomic studies to aid the development of diagnostics and therapeutics. We have sequenced, assembled and annotated the genome of A. cantonensis. The genome size is estimated to be ~260. Mb, with 17,280 genomic scaffolds, 91X coverage, 81.45% for complete and 93.95% for partial score based on CEGMA analysis of genome completeness. The number of predicted genes of ≥300. bp was 17,482. A total of 7737 predicted protein-coding genes of ≥50 amino acids were identified in the assembled genome. Among the proteins of known function, kinases are the most abundant followed by transferases. The draft genome contains 34 excretory-secretory proteins (ES), a minimum of 44 Nematode Astacin (NAS) metalloproteases, 12 Homeobox (HOX) genes, and 30 neurotransmitters. The assembled genome size (260. Mb) is larger than those of Pristionchus pacificus, Caenorhabditis elegans, Necator americanus, Caenorhabditis briggsae, Trichinella spiralis, Brugia malayi and Loa loa, but smaller than Haemonchus contortus and Ascaris suum. The repeat content (25%) is similar to H. contortus. The GC content (41.17%) is lower compared to P. pacificus (42.7%) and H. contortus (43.1%) but higher compared to C. briggsae (37.69%), A. suum (37.9%) and N. americanus (40.2%) while the scaffold N50 is 42,191. This draft genome will facilitate the understanding of many unresolved issues on the parasite and the disorder it causes.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalActa Tropica
Volume148
DOIs
Publication statusPublished - 1 Aug 2015
Externally publishedYes

Fingerprint

Angiostrongylus cantonensis
Parastrongylus cantonensis
Meningoencephalitis
meningoencephalitis
Parasites
Nematoda
Genome
parasites
Necator americanus
genome
Ascaris suum
Genome Size
Haemonchus contortus
Caenorhabditis briggsae
Caenorhabditis
Loa
Brugia malayi
genomics
Trichinella spiralis
Haemonchus

Keywords

  • Angiostrongylus cantonensis
  • Excretory-secretory proteins
  • Genome
  • Homeobox genes
  • Kinases
  • Metalloproteases
  • Neurotransmitters
  • Transferases

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Draft genome of neurotropic nematode parasite Angiostrongylus cantonensis, causative agent of human eosinophilic meningitis. / Yong, Hoi Sen; Eamsobhana, Praphathip; Lim, Phaik Eem; Mohamad Razali, Rozaimi; Aziz, Farhanah Abdul; Rosli, Nurul Shielawati Mohamed; Poole-Johnson, Johan; Anwar, Arif.

In: Acta Tropica, Vol. 148, 01.08.2015, p. 51-57.

Research output: Contribution to journalArticle

Yong, Hoi Sen ; Eamsobhana, Praphathip ; Lim, Phaik Eem ; Mohamad Razali, Rozaimi ; Aziz, Farhanah Abdul ; Rosli, Nurul Shielawati Mohamed ; Poole-Johnson, Johan ; Anwar, Arif. / Draft genome of neurotropic nematode parasite Angiostrongylus cantonensis, causative agent of human eosinophilic meningitis. In: Acta Tropica. 2015 ; Vol. 148. pp. 51-57.
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