Down-regulation of hypothalamic pro-opiomelanocortin (POMC) expression after weaning is associated with hyperphagia-induced obesity in JCR rats overexpressing neuropeptide y

Abdoulaye Diane, W. David Pierce, James C. Russell, C. Donald Heth, Donna F. Vine, Denis Richard, Spencer D. Proctor

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We hypothesised that hypothalamic feeding-related neuropeptides are differentially expressed in obese-prone and lean-prone rats and trigger overeating-induced obesity. To test this hypothesis, in the present study, we measured energy balance and hypothalamic neuropeptide Y (NPY) and pro-opiomelanocortin (POMC) mRNA expressions in male JCR:LA-cp rats. We compared, in independent cohorts, free-feeding obese-prone (Obese-FF) and lean-prone (Lean-FF) rats at pre-weaning (10 d old), weaning (21-25 d old) and early adulthood (8-12 weeks). A group of Obese-pair-feeding (PF) rats pair-fed to the Lean-FF rats was included in the adult cohort. The body weights of 10-d-old Obese-FF and Lean-FF pups were not significantly different. However, when the pups were shifted from dams' milk to solid food (weaning), the obese-prone rats exhibited more energy intake over the days than the lean-prone rats and higher body and fat pad weights and fasting plasma glucose, leptin, insulin and lipid levels. These differences were consistent with higher energy consumption and lower energy expenditure. In the young adult cohort, the differences between the Obese-FF and Lean-FF rats became more pronounced, yielding significant age effects on most of the parameters of the metabolic syndrome, which were reduced in the Obese-PF rats. The obese-prone rats displayed higher NPY expression than the lean-prone rats at pre-weaning and weaning, and the expression levels did not differ by age. In contrast, POMC expression exhibited significant age-by-genotype differences. At pre-weaning, there was no genotype difference in POMC expression, but in the weanling cohort, obese-prone pups exhibited lower POMC expression than the lean-prone rats. This genotype difference became more pronounced at adulthood. Overall, the development of hyperphagia-induced obesity in obese-prone JCR rats is related to POMC expression down-regulation in the presence of established NPY overexpression.

Original languageEnglish
Pages (from-to)924-932
Number of pages9
JournalBritish Journal of Nutrition
Volume111
Issue number5
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Fingerprint

Pro-Opiomelanocortin
Hyperphagia
Weaning
Neuropeptides
Down-Regulation
Obesity
Neuropeptide Y
Genotype
Adipose Tissue
Leptin
Energy Intake
Energy Metabolism
Young Adult
Fasting
Milk

Keywords

  • Homeostasis
  • Neuropeptides
  • Obese-prone animals
  • Weanling JCR rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Medicine(all)

Cite this

Down-regulation of hypothalamic pro-opiomelanocortin (POMC) expression after weaning is associated with hyperphagia-induced obesity in JCR rats overexpressing neuropeptide y. / Diane, Abdoulaye; Pierce, W. David; Russell, James C.; Heth, C. Donald; Vine, Donna F.; Richard, Denis; Proctor, Spencer D.

In: British Journal of Nutrition, Vol. 111, No. 5, 01.01.2014, p. 924-932.

Research output: Contribution to journalArticle

Diane, Abdoulaye ; Pierce, W. David ; Russell, James C. ; Heth, C. Donald ; Vine, Donna F. ; Richard, Denis ; Proctor, Spencer D. / Down-regulation of hypothalamic pro-opiomelanocortin (POMC) expression after weaning is associated with hyperphagia-induced obesity in JCR rats overexpressing neuropeptide y. In: British Journal of Nutrition. 2014 ; Vol. 111, No. 5. pp. 924-932.
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AU - Richard, Denis

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