Abstract
To evaluate the hypothesis that phorbol myristate acetate (PMA) might modulate the expression of the cystic fibrosis (CF) gene in epithelial cells, we examined the effect of PMA on CF mRNA levels and regulation of Cl- secretion. Strikingly, PMA down-regulated CF mRNA transcript numbers in a dose- and time-dependent manner. Importantly, in parallel with the reduction of CF mRNA levels, PMA-treated cells were unable to up-regulate Cl- secretion in a normal fashion in response to forskolin, an effect which was also dose- and time-dependent. Thus, PMA is capable of modulating expression of the CF gene and induces T84 cells to adopt the ''CF phenotype'' in regard to regulation of Cl- ion transport.
| Original language | English |
|---|---|
| Pages (from-to) | 10319-10323 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 266 |
| Issue number | 16 |
| Publication status | Published - 16 Aug 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis chloride secretory phenotype in epithelial cells by phorbol ester. / Trapnell, B. C.; Zeitlin, P. L.; Chu, C. S.; Yoshimura, K.; Nakamura, H.; Guggino, W. B.; Bargon, J.; Banks, T. C.; Dalemans, W.; Pavirani, A.; Lecocq, J. P.; Crystal, Ronald.
In: Journal of Biological Chemistry, Vol. 266, No. 16, 16.08.1991, p. 10319-10323.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Down-regulation of cystic fibrosis gene mRNA transcript levels and induction of the cystic fibrosis chloride secretory phenotype in epithelial cells by phorbol ester
AU - Trapnell, B. C.
AU - Zeitlin, P. L.
AU - Chu, C. S.
AU - Yoshimura, K.
AU - Nakamura, H.
AU - Guggino, W. B.
AU - Bargon, J.
AU - Banks, T. C.
AU - Dalemans, W.
AU - Pavirani, A.
AU - Lecocq, J. P.
AU - Crystal, Ronald
PY - 1991/8/16
Y1 - 1991/8/16
N2 - To evaluate the hypothesis that phorbol myristate acetate (PMA) might modulate the expression of the cystic fibrosis (CF) gene in epithelial cells, we examined the effect of PMA on CF mRNA levels and regulation of Cl- secretion. Strikingly, PMA down-regulated CF mRNA transcript numbers in a dose- and time-dependent manner. Importantly, in parallel with the reduction of CF mRNA levels, PMA-treated cells were unable to up-regulate Cl- secretion in a normal fashion in response to forskolin, an effect which was also dose- and time-dependent. Thus, PMA is capable of modulating expression of the CF gene and induces T84 cells to adopt the ''CF phenotype'' in regard to regulation of Cl- ion transport.
AB - To evaluate the hypothesis that phorbol myristate acetate (PMA) might modulate the expression of the cystic fibrosis (CF) gene in epithelial cells, we examined the effect of PMA on CF mRNA levels and regulation of Cl- secretion. Strikingly, PMA down-regulated CF mRNA transcript numbers in a dose- and time-dependent manner. Importantly, in parallel with the reduction of CF mRNA levels, PMA-treated cells were unable to up-regulate Cl- secretion in a normal fashion in response to forskolin, an effect which was also dose- and time-dependent. Thus, PMA is capable of modulating expression of the CF gene and induces T84 cells to adopt the ''CF phenotype'' in regard to regulation of Cl- ion transport.
UR - http://www.scopus.com/inward/record.url?scp=0025836232&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025836232&partnerID=8YFLogxK
M3 - Article
VL - 266
SP - 10319
EP - 10323
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 16
ER -