Dopamine affects the stability, hydration, and packing of protofibrils and fibrils of the wild type and variants of α-synuclein

Cristian Follmer, Luciana Romão, Carla M. Einsiedler, Thaís C.R. Porto, Flávio Alves Lara, Marlos Moncores, Gilberto Weissmüller, Hilal A. Lashuel, Peter Lansbury, Vivaldo Moura Neto, Jerson L. Silva, Debora Foguel

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Abstract

Parkinson's disease (PD) is characterized by the presence of cytoplasmic inclusions composed of a-synuclein (a-syn) in dopaminergic neurons. This suggests a pivotal role of dopamine (DA) on PD development. Here, we show that DA modulates differently the stability of protofibrils (PF) and fibrils (F) composed of wild type or variants of α-syn (A30P and A53T) as probed by high hydrostatic pressure (HHP). While in the absence of DA, all α-syn PF exhibited identical stability, in its presence, the variant-composed PF acquired a greater stability (DAPFwt < DAPFA30P = DAPF A53T), implying that they would last longer, which could shed light onto why these mutations are so aggressive. When α-syn was incubated for long times (18 days) in the presence of DA, we observed the formation of F by electronic microscopy, suggesting that the PF trapped in the presence of DA in short times can evolve into F. The stability of F was also altered by DA. DAFwt was more labile than Fwt, indicating that the former would be more susceptible to breakage. PFA30P and DAPF A30P, when added to mesencephalic and cortical neurons in culture, decreased the number and length of neurites and increased the number of apoptotic cells. Surprisingly, these toxic effects of PFA30P and DAPFA30P were practically abolished with HHP treatment, which was able to break the PF into smaller aggregates, as seen by atomic force microscopy. These results suggest that strategies aimed at breaking and/or clearing these aggregates is promising in alleviating the symptoms of PD.

Original languageEnglish
Pages (from-to)472-482
Number of pages11
JournalBiochemistry
Volume46
Issue number2
DOIs
Publication statusPublished - 16 Jan 2007

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Follmer, C., Romão, L., Einsiedler, C. M., Porto, T. C. R., Lara, F. A., Moncores, M., Weissmüller, G., Lashuel, H. A., Lansbury, P., Neto, V. M., Silva, J. L., & Foguel, D. (2007). Dopamine affects the stability, hydration, and packing of protofibrils and fibrils of the wild type and variants of α-synuclein. Biochemistry, 46(2), 472-482. https://doi.org/10.1021/bi061871+