DNA DAMAGE BINDING PROTEIN2 shapes the DNA methylation landscape

Catherine Schalk, Stéphanie Drevensek, Amira Kramdi, Mohamed Kassam, Ikhlak Ahmed, Valérie Cognat, Stéfanie Graindorge, Marc Bergdoll, Nicolas Baumberger, Dimitri Heintz, Chris Bowler, Pascal Genschik, Fredy Barneche, Vincent Colot, Jean Molinier

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. However, the factors at the nexus of DNA repair and chromatin modification/remodeling remain poorly characterized. Here, we uncover a previously unrecognized interplay between the DNA repair factor DNA DAMAGE BINDING PROTEIN2 (DDB2) and the DNA methylation machinery in Arabidopsis thaliana. Loss-of-function mutation in DDB2 leads to genome-wide DNA methylation alterations. Genetic and biochemical evidence indicate that at many repeat loci, DDB2 influences de novo DNA methylation by interacting with ARGONAUTE4 and by controlling the local abundance of 24-nucleotide short interfering RNAs (siRNAs). We also show that DDB2 regulates active DNA demethylation mediated by REPRESSOR OF SILENCING1 and DEMETER LIKE3. Together, these findings reveal a role for the DNA repair factor DDB2 in shaping the Arabidopsis DNA methylation landscape in the absence of applied genotoxic stress.

Original languageEnglish
Pages (from-to)2043-2059
Number of pages17
JournalPlant Cell
Volume28
Issue number9
DOIs
Publication statusPublished - 1 Sep 2016

Fingerprint

DNA methylation
DNA Methylation
DNA damage
DNA Damage
DNA repair
DNA Repair
DNA
Arabidopsis
Genome
genome
small interfering RNA
Chromatin Assembly and Disassembly
epigenetics
chromatin
eukaryotic cells
Eukaryota
Epigenomics
Arabidopsis thaliana
Small Interfering RNA
nucleotides

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Schalk, C., Drevensek, S., Kramdi, A., Kassam, M., Ahmed, I., Cognat, V., ... Molinier, J. (2016). DNA DAMAGE BINDING PROTEIN2 shapes the DNA methylation landscape. Plant Cell, 28(9), 2043-2059. https://doi.org/10.1105/tpc.16.00474

DNA DAMAGE BINDING PROTEIN2 shapes the DNA methylation landscape. / Schalk, Catherine; Drevensek, Stéphanie; Kramdi, Amira; Kassam, Mohamed; Ahmed, Ikhlak; Cognat, Valérie; Graindorge, Stéfanie; Bergdoll, Marc; Baumberger, Nicolas; Heintz, Dimitri; Bowler, Chris; Genschik, Pascal; Barneche, Fredy; Colot, Vincent; Molinier, Jean.

In: Plant Cell, Vol. 28, No. 9, 01.09.2016, p. 2043-2059.

Research output: Contribution to journalArticle

Schalk, C, Drevensek, S, Kramdi, A, Kassam, M, Ahmed, I, Cognat, V, Graindorge, S, Bergdoll, M, Baumberger, N, Heintz, D, Bowler, C, Genschik, P, Barneche, F, Colot, V & Molinier, J 2016, 'DNA DAMAGE BINDING PROTEIN2 shapes the DNA methylation landscape', Plant Cell, vol. 28, no. 9, pp. 2043-2059. https://doi.org/10.1105/tpc.16.00474
Schalk C, Drevensek S, Kramdi A, Kassam M, Ahmed I, Cognat V et al. DNA DAMAGE BINDING PROTEIN2 shapes the DNA methylation landscape. Plant Cell. 2016 Sep 1;28(9):2043-2059. https://doi.org/10.1105/tpc.16.00474
Schalk, Catherine ; Drevensek, Stéphanie ; Kramdi, Amira ; Kassam, Mohamed ; Ahmed, Ikhlak ; Cognat, Valérie ; Graindorge, Stéfanie ; Bergdoll, Marc ; Baumberger, Nicolas ; Heintz, Dimitri ; Bowler, Chris ; Genschik, Pascal ; Barneche, Fredy ; Colot, Vincent ; Molinier, Jean. / DNA DAMAGE BINDING PROTEIN2 shapes the DNA methylation landscape. In: Plant Cell. 2016 ; Vol. 28, No. 9. pp. 2043-2059.
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