DNA binding of regulatory factors interacting with MHC‐class‐I gene enhancer correlates with MHC‐class‐I transcriptional level in class‐I‐defective cell lines

O. Blanchet, J. F. Bourge, H. Zinszner, Zohreh Tatari Calderone, L. Degos, P. Paul

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Tumor cells frequently show a lack of surface Class‐I major histocompatibility complex (MHC) antigen expression. These molecules are key recognition structures for immune rejection of tumor cells and their absence at the surface of tumor cells could favor the progression of tumors. We have analyzed the transcriptional mechanisms that could lead to suppression of MHC‐Class‐I expression in human tumor cell K562. The expression of MHC‐Class‐I genes is highly controlled by regulatory factors interacting with an enhancer sequence upstream of MHC‐Class‐I genes. In this report we show that DNA binding activity of 2 regulatory factors, KBFI and NF‐KB, known to be essential for constitutive expression of MHC‐Class‐I genes, is deficient in nuclear extracts from K562 cells. Induction of class‐l gene expression at the surface of tumor cells by interferon‐γ (IFN‐γ) and tumor necrosis factor a (TNF‐α) shows that TNF‐a can act in synergy with IFN‐γ to induce DNA binding of both factors NF‐KB and KBFI to the Class‐I gene enhancer and that this induction of transcriptional factors is correlated with enhancement of MHC‐Class‐I mRNA transcription and cell‐surface antigen expression.

Original languageEnglish
Pages (from-to)138-145
Number of pages8
JournalInternational Journal of Cancer
Volume47
Issue number6 S
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Cell Line
DNA
Genes
Neoplasms
K562 Cells
Gene Expression
Interferons
Histocompatibility Antigens
Major Histocompatibility Complex
Tumor Necrosis Factor-alpha
Antigens
Messenger RNA

ASJC Scopus subject areas

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

DNA binding of regulatory factors interacting with MHC‐class‐I gene enhancer correlates with MHC‐class‐I transcriptional level in class‐I‐defective cell lines. / Blanchet, O.; Bourge, J. F.; Zinszner, H.; Tatari Calderone, Zohreh; Degos, L.; Paul, P.

In: International Journal of Cancer, Vol. 47, No. 6 S, 1991, p. 138-145.

Research output: Contribution to journalArticle

@article{7aca5b28832a4dbfb09aa67c7d2a75f1,
title = "DNA binding of regulatory factors interacting with MHC‐class‐I gene enhancer correlates with MHC‐class‐I transcriptional level in class‐I‐defective cell lines",
abstract = "Tumor cells frequently show a lack of surface Class‐I major histocompatibility complex (MHC) antigen expression. These molecules are key recognition structures for immune rejection of tumor cells and their absence at the surface of tumor cells could favor the progression of tumors. We have analyzed the transcriptional mechanisms that could lead to suppression of MHC‐Class‐I expression in human tumor cell K562. The expression of MHC‐Class‐I genes is highly controlled by regulatory factors interacting with an enhancer sequence upstream of MHC‐Class‐I genes. In this report we show that DNA binding activity of 2 regulatory factors, KBFI and NF‐KB, known to be essential for constitutive expression of MHC‐Class‐I genes, is deficient in nuclear extracts from K562 cells. Induction of class‐l gene expression at the surface of tumor cells by interferon‐γ (IFN‐γ) and tumor necrosis factor a (TNF‐α) shows that TNF‐a can act in synergy with IFN‐γ to induce DNA binding of both factors NF‐KB and KBFI to the Class‐I gene enhancer and that this induction of transcriptional factors is correlated with enhancement of MHC‐Class‐I mRNA transcription and cell‐surface antigen expression.",
author = "O. Blanchet and Bourge, {J. F.} and H. Zinszner and {Tatari Calderone}, Zohreh and L. Degos and P. Paul",
year = "1991",
doi = "10.1002/ijc.2910470725",
language = "English",
volume = "47",
pages = "138--145",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6 S",

}

TY - JOUR

T1 - DNA binding of regulatory factors interacting with MHC‐class‐I gene enhancer correlates with MHC‐class‐I transcriptional level in class‐I‐defective cell lines

AU - Blanchet, O.

AU - Bourge, J. F.

AU - Zinszner, H.

AU - Tatari Calderone, Zohreh

AU - Degos, L.

AU - Paul, P.

PY - 1991

Y1 - 1991

N2 - Tumor cells frequently show a lack of surface Class‐I major histocompatibility complex (MHC) antigen expression. These molecules are key recognition structures for immune rejection of tumor cells and their absence at the surface of tumor cells could favor the progression of tumors. We have analyzed the transcriptional mechanisms that could lead to suppression of MHC‐Class‐I expression in human tumor cell K562. The expression of MHC‐Class‐I genes is highly controlled by regulatory factors interacting with an enhancer sequence upstream of MHC‐Class‐I genes. In this report we show that DNA binding activity of 2 regulatory factors, KBFI and NF‐KB, known to be essential for constitutive expression of MHC‐Class‐I genes, is deficient in nuclear extracts from K562 cells. Induction of class‐l gene expression at the surface of tumor cells by interferon‐γ (IFN‐γ) and tumor necrosis factor a (TNF‐α) shows that TNF‐a can act in synergy with IFN‐γ to induce DNA binding of both factors NF‐KB and KBFI to the Class‐I gene enhancer and that this induction of transcriptional factors is correlated with enhancement of MHC‐Class‐I mRNA transcription and cell‐surface antigen expression.

AB - Tumor cells frequently show a lack of surface Class‐I major histocompatibility complex (MHC) antigen expression. These molecules are key recognition structures for immune rejection of tumor cells and their absence at the surface of tumor cells could favor the progression of tumors. We have analyzed the transcriptional mechanisms that could lead to suppression of MHC‐Class‐I expression in human tumor cell K562. The expression of MHC‐Class‐I genes is highly controlled by regulatory factors interacting with an enhancer sequence upstream of MHC‐Class‐I genes. In this report we show that DNA binding activity of 2 regulatory factors, KBFI and NF‐KB, known to be essential for constitutive expression of MHC‐Class‐I genes, is deficient in nuclear extracts from K562 cells. Induction of class‐l gene expression at the surface of tumor cells by interferon‐γ (IFN‐γ) and tumor necrosis factor a (TNF‐α) shows that TNF‐a can act in synergy with IFN‐γ to induce DNA binding of both factors NF‐KB and KBFI to the Class‐I gene enhancer and that this induction of transcriptional factors is correlated with enhancement of MHC‐Class‐I mRNA transcription and cell‐surface antigen expression.

UR - http://www.scopus.com/inward/record.url?scp=84988075091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84988075091&partnerID=8YFLogxK

U2 - 10.1002/ijc.2910470725

DO - 10.1002/ijc.2910470725

M3 - Article

C2 - 1906051

AN - SCOPUS:84988075091

VL - 47

SP - 138

EP - 145

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 6 S

ER -