DNA-based assays to distinguish date palm (arecaceae) gender

Maryam E. Al-Mahmoud, Eman Aldous, Eman K. Al-Azwani, Joel Malek

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Premise of the study: Date palm (Phoenix dactylifera) is one of the oldest cultivated trees and is critical to the development of arid land. The date palm is a dioecious monocot with separate male and female trees. This presents a challenge in development as it is impossible to distinguish trees until they flower approximately five to eight years after planting. Methods and Results: We have developed PCR-based assays capable of sex differentiation in multiple date palm cultivars. The primers are designed across gender-specific polymorphisms and demonstrated greater than 90% accuracy in distinguishing date palm gender across multiple varieties. Conclusions: These results indicate that the primers should be helpful in rapidly distinguishing date palm gender from the earliest stages that DNA can safely be collected. This is a vast savings in time over present approaches.

Original languageEnglish
JournalAmerican Journal of Botany
Volume99
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Arecaceae
Phoenix dactylifera
gender
assay
DNA
assays
savings
cultivar
flower
polymorphism
Sex Differentiation
sexual development
Liliopsida
arid lands
Phoeniceae
genetic polymorphism
planting
flowers
Polymerase Chain Reaction
cultivars

Keywords

  • Arecaceae
  • Date palm
  • Phoenix dactylifera
  • Sex determination
  • Sex-specific markers

ASJC Scopus subject areas

  • Plant Science
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

DNA-based assays to distinguish date palm (arecaceae) gender. / Al-Mahmoud, Maryam E.; Aldous, Eman; Al-Azwani, Eman K.; Malek, Joel.

In: American Journal of Botany, Vol. 99, No. 1, 01.2012.

Research output: Contribution to journalArticle

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