DNA at the entry-exit of the nucleosome observed by cryoelectron microscopy

Patrick Furrer, Jan Bednar, Jacques Dubochet, Ali Hamiche, Ariel Prunell

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the “entry-exit” region, a missing piece in our understanding of the chromatin fiber’s folding. The three-dimensional structure of “linker” DNA was investigated here on single nucleosomes reconstituted without H1 an a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 ± 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalJournal of Structural Biology
Volume114
Issue number3
DOIs
Publication statusPublished - May 1995

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ASJC Scopus subject areas

  • Structural Biology

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