DNA at the Entry-Exit of the Nucleosome Observed by Cryoelectron Microscopy

Patrick Furrer, Jan Bednar, Jacques Dubochet, Ali Hamiche, Ariel Prunell

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the "entry-exit" region, a missing piece in our understanding of the chromatin fiber's folding. The three-dimensional structure of "linker" DNA was investigated here on single nucleosomes reconstituted without H1 an a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 ± 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalJournal of Structural Biology
Volume114
Issue number3
DOIs
Publication statusPublished - May 1995
Externally publishedYes

Fingerprint

Cryoelectron Microscopy
Nucleosomes
DNA
Chromatin

ASJC Scopus subject areas

  • Structural Biology

Cite this

DNA at the Entry-Exit of the Nucleosome Observed by Cryoelectron Microscopy. / Furrer, Patrick; Bednar, Jan; Dubochet, Jacques; Hamiche, Ali; Prunell, Ariel.

In: Journal of Structural Biology, Vol. 114, No. 3, 05.1995, p. 177-183.

Research output: Contribution to journalArticle

Furrer, Patrick ; Bednar, Jan ; Dubochet, Jacques ; Hamiche, Ali ; Prunell, Ariel. / DNA at the Entry-Exit of the Nucleosome Observed by Cryoelectron Microscopy. In: Journal of Structural Biology. 1995 ; Vol. 114, No. 3. pp. 177-183.
@article{bfa6a00c18d447c89a6cd70fdf377bda,
title = "DNA at the Entry-Exit of the Nucleosome Observed by Cryoelectron Microscopy",
abstract = "Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the {"}entry-exit{"} region, a missing piece in our understanding of the chromatin fiber's folding. The three-dimensional structure of {"}linker{"} DNA was investigated here on single nucleosomes reconstituted without H1 an a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 ± 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region.",
author = "Patrick Furrer and Jan Bednar and Jacques Dubochet and Ali Hamiche and Ariel Prunell",
year = "1995",
month = "5",
doi = "10.1006/jsbi.1995.1017",
language = "English",
volume = "114",
pages = "177--183",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - DNA at the Entry-Exit of the Nucleosome Observed by Cryoelectron Microscopy

AU - Furrer, Patrick

AU - Bednar, Jan

AU - Dubochet, Jacques

AU - Hamiche, Ali

AU - Prunell, Ariel

PY - 1995/5

Y1 - 1995/5

N2 - Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the "entry-exit" region, a missing piece in our understanding of the chromatin fiber's folding. The three-dimensional structure of "linker" DNA was investigated here on single nucleosomes reconstituted without H1 an a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 ± 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region.

AB - Whereas the DNA path inside the nucleosome is well established, it is essentially unknown in the "entry-exit" region, a missing piece in our understanding of the chromatin fiber's folding. The three-dimensional structure of "linker" DNA was investigated here on single nucleosomes reconstituted without H1 an a 256-bp DNA fragment. Cryoelectron microscopy and three-dimensional reconstruction of the DNA axis reveal these nucleosomes as 1.61 ± 0.15 left-handed superhelical turn particles whose DNA arms bend away from the core particle, thus preventing the occurrence of a crossing in the entry-exit region.

UR - http://www.scopus.com/inward/record.url?scp=0029151148&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029151148&partnerID=8YFLogxK

U2 - 10.1006/jsbi.1995.1017

DO - 10.1006/jsbi.1995.1017

M3 - Article

VL - 114

SP - 177

EP - 183

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 3

ER -