Diurnal variation in orexin A immunoreactivity and prepro-orexin mRNA in the rat central nervous system

Shahrad Taheri, David Sunter, Catherine Dakin, Sasha Moyes, Leighton Seal, James Gardiner, Michela Rossi, Mohammad Ghatei, Stephen Bloom

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Orexins are a family of neuropeptides originally believed to be important mediators of food intake. The wide distribution of orexins and their receptors, however, has suggested other regulatory functions for these peptides including involvement in sleep and arousal mechanisms. In this study, we have demonstrated diurnal variation in orexin A immunoreactivity in the pons, from where locus coeruleus noradrenergic neurones innervate other brain areas to stimulate arousal, and in the preoptic/anterior hypothalamic region, an area implicated in the regulation of sleep and circadian rhythms. Orexin A immunoreactivity decreased by 50% in the preoptic/anterior hypothalamus from 09:00 to 21:00 h (P<0.0001), whilst in the pons, it increased by over 30% from 09:00 to 01:00 h (P=0.02). Prepro-orexin mRNA also displayed diurnal variation. This further suggests that orexins are involved in the regulation of the sleep/wake cycle.

Original languageEnglish
Pages (from-to)109-112
Number of pages4
JournalNeuroscience Letters
Volume279
Issue number2
DOIs
Publication statusPublished - 28 Jan 2000
Externally publishedYes

Fingerprint

Central Nervous System
Messenger RNA
Sleep
Pons
Arousal
Anterior Hypothalamic Nucleus
Anterior Hypothalamus
Adrenergic Neurons
Locus Coeruleus
Circadian Rhythm
Neuropeptides
Eating
Orexins
Peptides
Brain

Keywords

  • Arousal
  • Diurnal variation
  • Northern blot analysis
  • Orexin A
  • Prepro-orexin mRNA
  • Radioimmunoassay
  • Sleep

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Diurnal variation in orexin A immunoreactivity and prepro-orexin mRNA in the rat central nervous system. / Taheri, Shahrad; Sunter, David; Dakin, Catherine; Moyes, Sasha; Seal, Leighton; Gardiner, James; Rossi, Michela; Ghatei, Mohammad; Bloom, Stephen.

In: Neuroscience Letters, Vol. 279, No. 2, 28.01.2000, p. 109-112.

Research output: Contribution to journalArticle

Taheri, S, Sunter, D, Dakin, C, Moyes, S, Seal, L, Gardiner, J, Rossi, M, Ghatei, M & Bloom, S 2000, 'Diurnal variation in orexin A immunoreactivity and prepro-orexin mRNA in the rat central nervous system', Neuroscience Letters, vol. 279, no. 2, pp. 109-112. https://doi.org/10.1016/S0304-3940(99)00955-6
Taheri, Shahrad ; Sunter, David ; Dakin, Catherine ; Moyes, Sasha ; Seal, Leighton ; Gardiner, James ; Rossi, Michela ; Ghatei, Mohammad ; Bloom, Stephen. / Diurnal variation in orexin A immunoreactivity and prepro-orexin mRNA in the rat central nervous system. In: Neuroscience Letters. 2000 ; Vol. 279, No. 2. pp. 109-112.
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