Distribution and quantification of immunoreactive orexin A in rat tissues

Shahrad Taheri, Mehdi Mahmoodi, Jolanta Opacka-Juffry, Mohammad A. Ghatei, Stephen R. Bloom

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130 Citations (Scopus)

Abstract

A sensitive and specific radioimmunoassay for orexin A was developed. Orexin A immunoreactivity was found to be confined to the central nervous system (CNS) with the highest concentrations in the hypothalamus, inferior and superior colliculi and brainstem. Within the hypothalamus, the highest levels were found in the lateral and posterior hypothalamus. These regions had a greater orexin A content in females compared to males. The orexin A content of hypothalamic regions did not change with fasting and no difference was noted in hypothalami of rats fed a high fat diet. The hypothalamic orexin A content was not different in obese Zucker rats compared to lean controls. Thus, orexin A has a wide distribution in the CNS, but appetite regulation may not be its main function. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalFEBS Letters
Volume457
Issue number1
DOIs
Publication statusPublished - 20 Aug 1999

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Keywords

  • Central nervous system
  • Hypothalamus
  • Orexin A

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Taheri, S., Mahmoodi, M., Opacka-Juffry, J., Ghatei, M. A., & Bloom, S. R. (1999). Distribution and quantification of immunoreactive orexin A in rat tissues. FEBS Letters, 457(1), 157-161. https://doi.org/10.1016/S0014-5793(99)01030-3