Distinct effects of histone H1 and non-histone chromosomal proteins on the structure of nucleosomes and the chromatin fibre in solution

Stephan I. Dimitrov, Vladimir L. Makarov, Lyuben N. Marekov, Beltcho G. Beltchev

Research output: Contribution to journalArticle

Abstract

In this study we attempt to differentiate between the effects of the non-histone chromosomal proteins and histone H1 on the structure of the nucleosomes and the chromatin fibre in solution. The properties of chromatin preparations with different histone H1 and non-histone protein compositions were compared using circular dichroism and flow linear dichroism and the following conclusions were drawn. When histone H1 is absent the non-histone proteins partially prevent the unfolding of the nucleosomes at low ionic strength. The complete blocking of this unfolding, however, is accomplished only in the presence of histone H1. The non-histone proteins do not affect the orientation of the nucleosomes along the fibre axis. Only histone H1 can maintain the positive anisotropy of the chromatin fibre.

Original languageEnglish
Pages (from-to)122-124
Number of pages3
JournalInternational Journal of Biological Macromolecules
Volume7
Issue number2
DOIs
Publication statusPublished - 1985
Externally publishedYes

Fingerprint

Non Histone Chromosomal Proteins
Nucleosomes
Histones
Chromatin
Fibers
Proteins
Anisotropy
Dichroism
Circular Dichroism
Ionic strength
Osmolar Concentration
Chemical analysis

Keywords

  • chromatin fibre
  • histone H1
  • non-histone chromosomal proteins
  • nucleosome structure
  • Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Distinct effects of histone H1 and non-histone chromosomal proteins on the structure of nucleosomes and the chromatin fibre in solution. / Dimitrov, Stephan I.; Makarov, Vladimir L.; Marekov, Lyuben N.; Beltchev, Beltcho G.

In: International Journal of Biological Macromolecules, Vol. 7, No. 2, 1985, p. 122-124.

Research output: Contribution to journalArticle

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