Direct measurement of interactions between adsorbed protein layers using an atomic force microscope

W. Richard Bowen, Nidal Hilal, Robert W. Lovitt, Chris J. Wright

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

An atomic force microscope (AFM) in conjunction with the colloid probe technique has been used to measure directly the interaction of adsorbed layers of the protein bovine serum albumin (BSA). The BSA was adsorbed on both a silica colloid probe and a silica surface. Measurements of force- distance curves were made at various salt concentrations and pHs. The measured force-distance curves were in good quantitative agreement with predictions based on the DLVO theory using zeta potentials (outer Helmholtz plane potentials) calculated for BSA from an independently validated site- binding-site-dissociation surface model. The paper hence provides a direct confirmation that the AFM colloid probe technique can provide a useful means of directly quantifying the interaction of biological macromolecules.

Original languageEnglish
Pages (from-to)348-352
Number of pages5
JournalJournal of Colloid and Interface Science
Volume197
Issue number2
DOIs
Publication statusPublished - 15 Jan 1998
Externally publishedYes

Fingerprint

Colloids
Bovine Serum Albumin
Microscopes
albumins
microscopes
serums
proteins
Proteins
colloids
Silicon Dioxide
Silica
probes
interactions
Binding sites
Zeta potential
silicon dioxide
Macromolecules
curves
Salts
Binding Sites

Keywords

  • Atomic force microscope
  • BSA
  • Colloid probe
  • DLVO

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Direct measurement of interactions between adsorbed protein layers using an atomic force microscope. / Bowen, W. Richard; Hilal, Nidal; Lovitt, Robert W.; Wright, Chris J.

In: Journal of Colloid and Interface Science, Vol. 197, No. 2, 15.01.1998, p. 348-352.

Research output: Contribution to journalArticle

Bowen, W. Richard ; Hilal, Nidal ; Lovitt, Robert W. ; Wright, Chris J. / Direct measurement of interactions between adsorbed protein layers using an atomic force microscope. In: Journal of Colloid and Interface Science. 1998 ; Vol. 197, No. 2. pp. 348-352.
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