Direct grafting of RGD-motif-containing peptide on the surface of polycaprolactone films

Matthias Gabriel, Geerten P. Van Nieuw Amerongen, Victor W M Van Hinsbergh, Arie V. Van Nieuw Amerongen, Andrej Zentner

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Direct surface modification of biodegradable polycaprolactone (PCL) was performed without the necessity of synthesis of functionisable co-polymers. An easy-to-perform three-step procedure consisting of amination, reaction with hetero-bifunctional cross-linkers and conjugation of an RGD-motif-containing peptide was used to modify polymer films and improve the attachment of endothelial cells. The biological activity of modified surfaces was assessed by estimating microvascular endothelial cell attachment. Covalent coating with RGD resulted in an approximately 11-fold increase of endothelial cell attachment on modified PCL surfaces compared with untreated polymer. The specificity of the attachment enhancement was confirmed by using a control peptide. It is concluded that chemical surface modification is an appropriate method of rendering degradable polymers, such as PCL, cell-adhesive.

Original languageEnglish
Pages (from-to)567-577
Number of pages11
JournalJournal of Biomaterials Science, Polymer Edition
Volume17
Issue number5
DOIs
Publication statusPublished - May 2006
Externally publishedYes

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Keywords

  • Biodegradation
  • Cell attachment
  • Polycaprolactone
  • RGD motif
  • Surface modification

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials

Cite this

Gabriel, M., Van Nieuw Amerongen, G. P., Van Hinsbergh, V. W. M., Van Nieuw Amerongen, A. V., & Zentner, A. (2006). Direct grafting of RGD-motif-containing peptide on the surface of polycaprolactone films. Journal of Biomaterials Science, Polymer Edition, 17(5), 567-577. https://doi.org/10.1163/156856206776986288